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相关概念视频

Xylem and Transpiration-driven Transport of Resources02:03

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Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
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Assaying Proteasomal Degradation in a Cell-free System in Plants
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活性蛋白的无处不在调节了体血管的功能.

Pawittra Phookaew1, Ya Ma2, Takaomi Suzuki1

  • 1Graduate School of Science and Technology, Division of Biological Science, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.

The Plant cell
|August 2, 2024
PubMed
概括
此摘要是机器生成的。

蛋白质无化调节了体血管的发育. 与血管相关的NAC-DOMAIN7 (VND7) 的特定无处不在对于其活性和植物中的正确质功能至关重要.

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科学领域:

  • 植物生物学 植物生物学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 体容器对于植物中的水运输至关重要.
  • 与血管相关的NAC-DOMAIN7 (VND7) 是体血管细胞分化的一个关键调节器.

研究的目的:

  • 为了确定参与调节VND7诱导的体血管细胞分化的基因.
  • 阐明蛋白质无化在树发育中的作用.

主要方法:

  • 由VND7 (seiv) 突变体诱导的异位细胞细胞分化的抑制剂的分离和表征.
  • 对基因表达的分析,蛋白质泛化水平和VND7的交换活化活动.

主要成果:

  • 鉴定出seiv3,seiv4,seiv6和seiv9突变基因是参与蛋白质无化 (PUB46,FBX,PUB36,UBP1) 的基因.
  • seiv突变体表现出下游基因表达的减少和体运输的受损.
  • 诱导VND7导致了细胞壁生物合成和运输中涉及的蛋白质的改变无处不在.
  • 在VND7中检测到lysine残留物K94,K105和K260的ubiquitination,其中K94的ubiquitination对VND7活性至关重要.

结论:

  • 蛋白质无化是体血管发育中的关键调节机制.
  • 在VND7上,特别是K94上,特定的无处不在位点对于其调节体分化的功能至关重要.