在治疗肝癌时Hedyotis Diffusae Herba的免疫疗法机制:基于网络药理学,生物信息学和实验验证的研究
Qingsheng Zheng1, Xueying Wu1, Shuai Peng2
1Department of General Surgery, The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine, 602 Ba Yi Qi Zhong Road, Taijiang District, Fuzhou, 350108, Fujian, China.
威尔德 (Hedyotis diffusa Willd,HDW) 通过准IL6和TNF,显示出对抗肝癌的抗瘤作用. 这种中国传统医学的成分,素,抑制癌细胞的生长,并通过特定的信号通路促进细胞亡.
科学领域:
- 综合性瘤学是一种综合性瘤学.
- 网络药理学 网络药理学
- 生物信息学是一种生物信息学.
背景情况:
- 肝癌是全球重要的健康问题.
- 威尔德 (HDW) 扩散虫表现出抗瘤特性,但其在肝癌中的机制尚未完全理解.
- 了解HDW的免疫治疗机制对于开发新的肝癌治疗方法至关重要.
研究的目的:
- 为了阐明HDW对抗肝癌的免疫治疗机制.
- 通过网络药理学和生物信息学来识别HDW的活性成分,潜在目标和分子途径.
- 通过实验方法验证发现.
主要方法:
- 网络药理学 (TCMSP,STRING,Cytoscape) 以确定HW目标并构建蛋白质-蛋白质相互作用网络.
- 生物信息学分析 (TCGA,HPA) 来评估基因表达,生存和免疫透.
- 分子对接,MTT测定,流细胞计,RT-qPCR和西班牙血栓用于实验验证.
主要成果:
- 确定了163个潜在目标,其中IL6和TNF是核心目标.
- 凯格分析显示了AGE-RAGE,IL-17,TNF,PI3K-Akt和NF-κB信号通路的参与.
- 奎尔赛丁,一个核心的HDW成分,抑制了肝癌细胞的增殖,促进了细胞亡,并降低了IL6和TNF的表达.
结论:
- HDW通过多个点和信号通路发挥抗癌作用,特别是涉及IL6和TNF.
- 奎尔是调解这些效应的关键活性成分.
- 这项研究为HDW作为肝癌的潜在免疫治疗剂提供了科学依据.
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