昆虫传播的植物病毒通过调节Rab1介导的受体局部化来平衡其垂直传播
Qing Liu1, Xiangyi Meng1, Zhiyu Song1
1State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
Cell reports
|August 2, 2024
概括
条病毒 (RSV) 通过阻断维特洛根因受体通路,延迟了昆虫卵巢的成熟. 这种病毒策略影响了卵子的发育,并提供了对垂直病毒传播的见解.
科学领域:
- 病毒学 病毒学
- 昆虫分子生物学 昆虫分子生物学
- 生殖生物学 生殖生物学
背景情况:
- 条病毒 (RSV) 感染其载体的卵巢,植物 Laodelphax striatellus.
- 病毒感染可以破坏宿主生殖过程,影响传播效率.
研究的目的:
- 阐明RSV延迟L.striatellus.卵巢成熟的分子机制.
- 为了确定参与RSV生殖操纵的宿主因素.
主要方法:
- 研究了RSV核蛋白 (NP) 和宿主因子之间的相互作用.
- 利用技术来评估蛋白质局部化,GTP结合和水解.
- 检查了宿主蛋白LsRab1在维氏原蛋白受体 (VgR) 贩运和维氏原蛋白 (Vg) 积累中的作用.
主要成果:
- RSV感染通过防止VgR膜局部化来抑制卵巢成熟,从而减少Vg积累用于卵子发育.
- 主体蛋白质LsRab1在护士细胞中直接与RSV NP相互作用.
- RSV NP通过阻止GTP结合和刺激GTP水解来抑制LsRab1的功能,形成一个不活跃的LsRab1.
- 这种双重抑制阻止LsRab1将VgR贩运到细胞膜,导致Vg吸收效率低下.
结论:
- RSV采用了针对LsRab1的双重抑制策略,以破坏VgR贩运和延迟卵巢成熟.
- 了解这种机制,可以了解RSV和潜在的其他昆虫传播病毒的垂直传播.
- 保存的Vg-VgR通路对研究卵性动物病毒与宿主相互作用具有更广泛的意义.
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