通过定量PCR在唾液中检测Mycobacterium结核复合体:一个潜在的替代标本肺结核诊断肺结核
Sosina Ayalew1, Teklu Wegayehu2, Binium Wondale2
1Armauer Hansen Research Institute, Addis Ababa, Ethiopia; Department of Biology, College of Natural and Computational Sciences, Arba Minch University, Arba Minch, Ethiopia.
Tuberculosis (Edinburgh, Scotland)
|August 2, 2024
概括
唾液检测显示,它可以作为诊断肺结核 (PTB) 的唾液替代品. 这种方法提供了一个可行的选择,当唾液收集对患者来说是具有挑战性的.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 诊断医学 诊断医学
背景情况:
- 基于唾液的诊断是结核病 (TB) 的标准,但对一些患者来说具有挑战性.
- 肺结核 (PTB) 诊断需要可访问和可靠的方法.
研究的目的:
- 评估唾液作为PTB的替代诊断样本.
- 评估用于结核病检测的唾液定量PCR (qPCR) 的性能.
主要方法:
- 在唾液样本上使用了针对IS1081基因的定量PCR (qPCR).
- 该研究包括32名PTB患者和30名患有其他呼吸道疾病 (ORD) 的患者.
主要成果:
- 唾液IS1081 qPCR显示PTB检测的灵敏度为65.6%,特异性为96.7%.
- 与sputum-Xpert.相比,灵敏度提高到72.4%,与sputum-Xpert.相比.
- 接收器操作特征 (ROC) 分析显示曲线下的面积 (AUC) 为82.5%.
结论:
- 唾液检测是诊断PTB的唾液的潜在替代方案.
- 需要进一步的多中心研究来确认不同患者群体的诊断准确性.
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