相关实验视频
Updated: Jun 18, 2025

09:05
MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
7.5K
瘟病毒编码的微RNA dev-miR-D28-3p通过准UL27来抑制病毒复制
Hui Ni1, Xingcui Zhang2, Juan Huang3
1Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang District, Chengdu City, Sichuan Province 611130, China.
Veterinary microbiology
|August 2, 2024
概括
瘟疫病毒 (DPV) 微RNAs (miRNAs) 调节病毒复制. Dev-miR-D28-3p通过准UL27基因来抑制DPV复制,揭示了一个新的病毒调节机制.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 基因规则 基因规则
背景情况:
- 疹病毒微RNAs (miRNAs) 是病毒生命周期的关键调节者.
- 由瘟疫病毒 (DPV) 编码的miRNAs的功能在很大程度上仍未被探索.
研究的目的:
- 为了研究 dev-miR-D28-3p,一个DPV编码的miRNA 在DPV复制中的作用.
- 阐明dev-miR-D28-3p介导的病毒调节的潜在分子机制.
主要方法:
- 在子胚胎纤维细胞 (DEFs) 中转移dev-miR-D28-3p模仿和抑制剂.
- 病毒副本,标位和蛋白质表达的量化.
- 使用结合试验确定dev-miR-D28-3p的目标基因.
- 对目标基因UL27.7的过度表达和淘汰研究.
主要成果:
- Dev-miR-D28-3p模仿转染抑制了DPV复制,而抑制转染则逆转了这种效应.
- 发现Dev-miR-D28-3p直接结合并抑制DPV编码UL27.7的表达.
- UL27过度表达增强了DPV复制,而UL27敲击抑制了它.
结论:
- 通过准UL27基因,DPV利用dev-miR-D28-3p来调节自己的复制.
- 这项研究揭示了DPV中一种新的miRNA介导的自我调节机制.
- 这些发现为进一步研究DPV编码的miRNAs提供了基础.
相关概念视频
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
siRNA - Small Interfering RNAs
16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K

