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类水果中的LED蓝光对荷尔蒙代谢的影响
María T Lafuente1, Raúl Sampedro1, Paco Romero1
1Department of Food Biotechnology, Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Avenida Dr. Catedrático Agustín Escardino 7, 46980, Paterna, Valencia, Spain.
Plant physiology and biochemistry : PPB
|August 2, 2024
概括
在子中,LED蓝光 (LBL) 治疗显著改变了激素水平和莉酸盐 (JA) 代谢. LBL影响莉酸 (JA) 和其前体OPDA,影响水果防御通路,并可能减少收获后腐烂.
科学领域:
- 植物生理学 植物生理学
- 收获后生物学 收获后生物学
- 园艺科学 园艺科学
背景情况:
- 收获后的子腐烂,主要是由*Penicillium digitatum*引起的,导致了重大的经济损失.
- 斯酸盐 (JAs) 是关键的植物激素,参与对病原体的防御反应.
- LED蓝光 (LBL) 是作为一种非化学方法来提高水果质量和减少腐败的探索.
研究的目的:
- 为了研究LED蓝光 (LBL) 对子激素水平和莉酸盐 (JA) 代谢的影响.
- 了解 LBL 诱导植物激素通路的变化背后的分子机制.
- 评估LBL在管理收获后疾病方面的潜力.
主要方法:
- 子用LED蓝光 (450 nm,60 μmol m-2 s-1 2天) 进行处理.
- 分析关键的植物激素,包括酸 (ABA),酸 (SA),醇-3-酸 (IAA) 和斯酸 (JAs) 以及它们的代谢物.
- 对参与JA生物合成和信号通路的酶的基因表达分析 (例如,CsLOX,CsAOC,CsOPR,CsACX,COI1,CsJAZ).
主要成果:
- LBL显著改变了子中ABA,SA,IAA和JA的含量.
- 观察到对莉酸 (JA) 和其前体cis-(+) -12-oxo-phytodienoic acid (OPDA) 水平的主要影响.
- 在OPDA和JA生物合成中参与的LBL上调基因 (CsLOX1,CsLOX5,CsAOC3,CsOPR2,CsACX1,CsACX3).
- 通过增加COI1受体表达和降低CsJAZ抑制剂的调节,LBL增强了JA感知和信号传递.
- 当处理过的水果转移到黑暗中时,由LBL引起的荷尔蒙变化减少了,但IAA和SA水平略有增加.
结论:
- 在子中,LED蓝光 (LBL) 有效调节植物激素代谢,特别是莉花激素的代谢.
- LBL影响JA生物合成和信号通路中的关键基因,这表明它在增强水果防御方面发挥了作用.
- 这些发现提供了关于LBL作为提高果收获后质量和抗病能力的工具的潜力的见解.
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