盐和低酸盐应激条件的结合导致了番茄的形态生理变化和组织特异性的转录组重编程
Giorgia Batelli1, Alessandra Ruggiero1, Salvatore Esposito1
1National Research Council of Italy, Institute of Biosciences and BioResources, Research Division Portici (CNR-IBBR), Portici, 80055, Italy.
Plant physiology and biochemistry : PPB
|August 2, 2024
概括
盐和低酸盐压力的结合显著影响番茄的生长,产量和果实大小. 叶子的基因表达主要反映了低酸盐,而根则对盐更敏感,揭示了对联合无生物应激的特定分子反应.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 番茄的非生物应激适应对作物弹性至关重要.
- 了解盐和低酸盐 (低N) 的压力效应是有限的.
- 番茄基因型对环境挑战表现出不同的反应.
研究的目的:
- 在单一和组合盐和低N压力下调查番茄的生理和转录组变化.
- 为了识别叶子和根的组织特异性分子反应.
- 为了揭示参与适应联合非生物压力的基因.
主要方法:
- 在受控的单一和组合应激条件下种植三种传统番茄基因型.
- 综合生理,生长和产量测量.
- mRNA测序 (RNA-Seq) 用于根和叶的转录组分析.
- 基因本体学 (GO) 和基因和基因组 (KEGG) 丰富分析的京都百科全书.
主要成果:
- 结合盐和低N压力导致了显著的形态生理学修饰,导致产量和水果大小的最大减少.
- 在组合应激下,叶子转录组在很大程度上反映了低N效应,而根转录组对盐应激高度敏感.
- 功能丰富分析证实了压力和组织特异性的基因表达模式.
- 鉴定了与核糖体生物发生和叶子中的酸盐运输以及根中的应激反应/运输相关的新型基因,这些基因特定于组合应激条件.
结论:
- 盐和低压力对番茄的生长和生产率造成了严重的损害.
- 番茄表现出独特的组织特异性转录基因对盐和低N的反应,无论是单独的还是组合的.
- 这项研究提供了全面的分子和生理学了解番茄对非生物应激的反应,为改善作物表现的育种策略提供了信息.
相关概念视频
02:02Responses to Salt Stress
13.1K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.1K
02:45Responses to Heat and Cold Stress
13.4K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.4K
02:35Key Elements for Plant Nutrition
18.7K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
18.7K
02:04Regulation of Transpiration by Stomata
28.1K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.1K
01:10Transcription
146.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
146.8K