细胞大小通过依赖于actomyosin的AMOT-YAP信号调节人体内皮的特异性
Lai Jiang1, Chenchao Yan2, Ying Yi2
1State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Science, Hubei University, Wuhan 430062, China; Department of Biological Repositories, Frontier Science Center for Immunology and Metabolism, Medical Research Institute, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan 430071, China.
细胞大小的减少增强了人类多能干细胞的分化到最终的内皮. 这一过程涉及actomyosin活性,血管蛋白核转位和抑制的YAP信号,为内皮系诱导提供了一种新方法.
科学领域:
- 细胞生物学 细胞生物学
- 发育生物学是发展生物学.
- 生物物理学的生物物理.
背景情况:
- 细胞大小是影响细胞功能的基本物理性质.
- 细胞大小动态在干细胞规范中的作用尚不清楚.
- 人类多能干细胞 (hPSCs) 分化成各种细胞类型,包括最终内皮 (DE).
研究的目的:
- 为了研究hPSC分化成DE的过程中细胞大小的动态变化.
- 探索细胞大小调制对DE分化的影响.
- 阐明将细胞大小缩小与DE规范联系起来的分子机制.
主要方法:
- 在hPSC-to-DE分化过程中监测细胞大小变化.
- 施加高压和化学处理以诱导细胞大小的减少.
- 研究机械敏感元件的作用,包括actomyosin,AMOT和YAP.
- 使用活细胞成像和生物化学分析.
主要成果:
- 细胞大小在DE分化过程中逐渐减少,特别是在更硬的基板上.
- 通过高压或化学方法加速细胞大小的缩小,意味着显著增强DE分化.
- 鉴定出actomyosin活性是细胞大小减少和DE分化的关键调解者.
- 细胞大小的减少促进了AMOT核转移,抑制了YAP活动并促进了DE的分化.
结论:
- 在hPSCs中,细胞大小减少和最终的内皮分化之间建立了新的联系.
- 该机制涉及依赖于actomyosin的AMOT核转位和随后的YAP抑制.
- 透压是一种有前途的策略,可以促进人类内皮系的分化.
更多相关视频
12:35Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
Published on: April 14, 2023
08:06A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis
Published on: March 19, 2021
相关概念视频
Cells Coordinate Growth and Proliferation
Cell Motility through Blebbing
Blebbing Through the Matrix
In multicellular...
The Contractile Ring
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Actin Polymerization and Cell Motility
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
The Role of Actin and Myosin in Non-muscle Cells
