相关实验视频

Updated: Jun 18, 2025

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

辅助性NRC4免疫受体的激活形成了一个六美体抵抗体

Furong Liu¹, Zhenlin Yang², Chao Wang³

¹Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.

|August 2, 2024

概括

植物免疫受体,核酸结合性氨酸丰富的重复受体 (NLRs),协调对抗病原体. 像NRC4这样的辅助NLRs (hNLRs) 形成抵抗体,触发流入和植物免疫力.

科学领域:

植物免疫力植物免疫力
分子生物学分子生物学
蜂信号传输是如何进行的

背景情况:

植物和动物的先天免疫依赖于核酸结合的氨酸丰富的重复受体 (NLR).
助手NLRs (hNLRs) 与传感器NLRs (sNLRs) 在植物疾病抵抗路径中合作.
高NLRs的结构激活机制在很大程度上仍然没有特征.

研究的目的:

为了阐明植物hNLR的结构激活机制,NRC4.
研究NRC4在植物免疫信号传递中的作用.
探索调节NRC激活的特定植物因素.

主要方法:

对NRC4激活的结构分析.
生物化学试验用于研究蛋白质复合体形成 (抵抗体).
在植物和动物细胞中测量离子 (Ca2+) 流入量.

主要成果:

在sNLR Bs2和效应器AvrBs2.2激活后,NRC4组装成一个六美体抗体.
通过NRC4形成的抗体会触发Ca2+的涌入,从而启动免疫反应.
激活模仿NRC2,NRC3或NRC4的等位基因并没有诱导动物细胞中的Ca2+流入或细胞死亡.

结论:

该研究揭示了植物中hNLR激活的结构基础,涉及抵抗体的形成.
激活NRC4是植物先天免疫的一个关键步骤,由Ca2+流入介导.
植物特异性因素对NRC激活至关重要,突出了植物和动物免疫系统之间的差异.

关键词:

欧洲资本贸易协会 (ETI)它们是NLR蛋白质.在NRC0中,它是NRC0.在NRC2中,它是NRC2.在NRC3中,它是NRC3.在 NRC4 抗体组中,NRC4 抗体组具有抗性.这是PTI PTI.的流入的流入.病原体识别病原体识别植物免疫力植物免疫力

更多相关视频

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

相关概念视频

Protein Complex Assembly

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

Rab Proteins

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...

Diversity of Antigen Receptors

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

Rab Cascades

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.

Cooperative Binding of Transcription Regulators

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...