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从废物活性污泥发酵系统中通过乳杆菌注射产生碳酸盐和酒精:洞察过程结果和代谢网络

Lan Wu¹, Huu Hao Ngo¹, Chen Wang²

¹Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia.

Bioresource technology

|August 2, 2024

概括

这项研究发现,废弃活性污泥 (WAS) 中较低的乳酸菌注射最大限度地提高了中链脂肪酸 (MCFA) 的产量. 较高的接种率有利于酒精生成,为从废物中获得可持续生物能源提供了洞察力.

科学领域:

生物技术是生物技术.
环境科学环境科学
化学工程是化学工程的重要组成部分.

背景情况:

废物活性污泥 (WAS) 是一个丰富的未充分利用的资源.
化学工业对中链脂肪酸 (MCFA) 的可持续生产至关重要.
有效地将WAS转化为有价值的产品仍然是一个挑战.

研究的目的:

调查乳杆菌注射度对WAS.MCFAs产生的影响.
要了解碳流向MCFA和酒精的重新定向.
确定影响发酵结果的微生物和遗传因素.

主要方法:

用不同度的乳酸菌 (7.94 × 10^10至6.35 × 10^11细胞/L) 接种WAS.
对MCFA和酒精生产的分析.
微生物社区分析 (Clostridium主导地位) 和基因丰度分析.

主要成果:

最高的MCFA产量 (∼2000毫克COD/L) 是通过最低的Lactobacillus注射 (7.94 × 10^10细胞/L) 实现的.
较高的乳酸菌度 (高达6.35×10^11细胞/L) 导致酒精产量增加 (高达2852毫克COD/L).
乳杆菌注射调节了微生物群落和基因表达,增强了基质水解和产品合成.

结论:

乳酸菌注射是一种可行的策略,可以调节WAS的发酵途径.
优化乳杆菌度控制了MCFA和酒精生产之间的平衡.
这种方法提供了一种可持续的方法,用于从WAS中回收生物能源.

关键词:

链条的延长链条的延长液体产品是一种液体产品.超基因组学分析自己供应的乳酸盐.

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