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Updated: Jun 18, 2025

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基于代谢学的反向代谢工程,用于Saccharomyces cerevisiae有效地生产氧

Yingjie Liu1, Bixuan Gu1, Jiahua Shi2

  • 1School of Food Science and Technology, Jiangnan University, 214122 Wuxi, China.

Bioresource technology
|August 2, 2024
PubMed
概括

代谢工程被用来改善酵母中的氧醇产量. 反向代谢工程确定并解决了限制速率的步骤,显著提高了产量超过118%.

关键词:
氧铁醇的使用方法逆代谢工程是一种逆代谢工程.代谢组分析分析它们中的一种是S. cerevisiae.

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Fates of Pyruvate01:20

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Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
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