暴露在微塑料中会破坏生,并诱导人类iPSC衍生器官的毒性
Bingrui Zhou1, Yunliang Wei1, Long Chen1
1Department of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, MOE Key Laboratory of Coal Environmental Pathogenicity and Prevention, Shanxi Medical University, Taiyuan, 030001, China.
Environmental pollution (Barking, Essex : 1987)
|August 2, 2024
概括
微塑料 (MP) 损害了人类早期脏发育. 暴露在器官中的聚乙烯MPs导致了体型的缩小,异常结构,并破坏了细胞信号传递,影响了生.
科学领域:
- 环境健康 环境健康
- 发展生物学 发展生物学
- 毒理学 毒理学 毒理学
背景情况:
- 微塑料 (MP) 是在人体组织中检测到的全球污染物,引起了对胎儿发育的担忧.
- 已知MPs对成年人造成损伤,但对早期发育 (发育) 的影响尚不清楚.
- 需要人类脏器官模型来研究MP对产生的影响.
研究的目的:
- 使用体外有机体 (in vitro organoids) 研究微塑料对人类发育的影响.
- 确定微塑料在细胞和分子层面影响产生的机制.
主要方法:
- 在原细胞 (NPC) 阶段,人类器官被暴露在聚乙烯微塑料 (PS-MP) 中.
- 对有机体的顺序检查评估了对NPC,囊和逗号形体阶段的影响.
- 分析包括器官体大小,脏结构,活性氧物种 (ROS) 生产,细胞亡,细胞活力和转录基因概况 (Notch信号).
主要成果:
- PS-MPs附着在细胞中,并积聚在类似质体的结构中.
- 暴露导致了较小的有机体尺寸和异常的脏结构.
- 暴露于PS-MP增加了ROS,诱导了NPC亡,降低了细胞活力,降低了NPC数量.
- 转录组分析显示,诺奇信号降低调节,破坏管状结构和脏模式.
结论:
- 聚乙烯微塑料显著破坏人类脏发育.
- PS-MPs通过包括ROS产生,亡和Notch信号通路中断在内的机制诱导脏毒性.
- 这项研究提供了关于微塑料对早期发育的不良影响的关键见解.
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