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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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系统性功能丧失屏幕识别特定途径的功能循环RNA.

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概括

这项研究确定了调节细胞通路的功能循环RNAs (circRNAs). 研究人员选了成千上万的人类circRNAs,以发现癌细胞增殖和信号通路的新型调节者.

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 癌症研究 癌症研究

背景情况:

  • 循环RNAs (circRNAs) 是一种由反向拼接形成的RNA分子类.
  • 虽然一些circRNAs具有已知的功能,但它们调节的途径在很大程度上仍未被探索.
  • 了解circRNA功能对于破译复杂的细胞过程至关重要.

研究的目的:

  • 为了确定功能性circRNA和它们调节的途径.
  • 研究circRNAs在癌细胞增殖和信号传递中的作用.
  • 创建具有可测量的功能的circRNAs目录.

主要方法:

  • 创建了一个聚合的短毛RNA库,针对3,354个人类circRNAs.
  • 在四种组织类型的18个癌细胞系中进行了功能丧失增殖查.
  • 通过二次查验证的发现,并确定了特定的circRNA调节器.

主要成果:

  • 确定了特定的和非特定的功能性circRNAs.
  • 发现了调节自身前体和具有独立功能的circRNAs.
  • 验证了关键的circRNAs,包括circRERE,circHUWE1,circSMAD2 (WNT通路) 和circMTO1 (MAPK信号传递).

结论:

  • 这项研究提供了关于circRNA介导途径调节的见解.
  • 已经建立了参与癌细胞生物学的功能性circRNAs的目录.
  • 这项研究突出了circRNAs在细胞过程中的多样性作用.