在德国和丹麦的鹿类中蛋白基因 (PRNP) 变异
Sonja Ernst1, Agata Piestrzyńska-Kajtoch2, Jörn Gethmann3
1Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Greifswald, Isle of Riems, Germany.
Veterinary research
|August 2, 2024
概括
欧洲鹿,特别是红鹿的蛋白基因 (PRNP) 变异显示出显著的遗传多样性. 这种PRNP变异对于了解慢性衰竭疾病 (CWD) 易感性和管理风险鹿种群至关重要.
科学领域:
- 兽医科学 兽医科学 兽医科学
- 遗传学 遗传学 是一个
- 野生动物疾病生态学 野生动物疾病生态学
背景情况:
- 细胞蛋白 (PrP) 结构,由蛋白基因 (PRNP) 编码,影响易受传染性海绵状脑病变 (TSEs) 的易感性.
- 慢性衰竭疾病 (CWD),一种TSE,已经在欧洲鹿种中出现,反映了北美疫情.
- 研究欧洲鹿的PRNP变异对于风险评估和CWD管理策略至关重要.
研究的目的:
- 为了分析PRNP序列变异在德国红鹿,鹿,锡卡和休鹿,以及丹麦红鹿和动物园物种.
- 确定PRNP基因型及其在欧洲鹿种群中的地理分布.
- 根据PRNP变异评估欧洲鹿类对CWD的潜在易感性.
主要方法:
- 从911个德国鹿和26个丹麦红鹿的样本中测序PRNP基因.
- 来自四种非原生动物园物种的PRNP序列数据的分析.
- 鉴定到的PRNP多态和突变的基因型和地理分析.
主要成果:
- 在鹿和鹿中没有发现PRNP序列变异.
- 在红鹿中检测到显著的PRNP变异,包括非同义多态 (codons 98,226,247),同义突变 (codons 21,78,136,185),以及在八重复区域的新24bp删除.
- 确定了14种不同的红鹿基因型,表现出显著的地理分布差异和集群,表明"PRNP血统".
结论:
- 欧洲红鹿表现出相当大的PRNP遗传多样性,具有明显的地理模式.
- 在欧洲鹿中发现的PRNP变异可能会影响它们对CWD的易感性.
- 欧洲有很大一部分野生鹿类如果被引入原始种群,可能会对CWD敏感.
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