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与年龄相关的线粒细胞衰变通过影响PDLSCs线粒体功能和RANKL/OPG的作用来调节正义牙的移动
Tong Yan1,2,3, Huilin Li1,2,3, Jiayin Yan1,2,3
1Department of Orthodontics, Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, China.
概括
线性,一个细胞过程,在正统牙牙运动期间,对于骨重塑至关重要. 与年龄相关的线粒衰减会损害这一过程,影响治疗结果.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 矯正牙科 矯正牙科是一種矯正牙科.
背景情况:
- 线对于骨代谢和与年龄有关的疾病至关重要.
- 它在正牙移动 (OTM) 和与年龄相关的骨重塑中的作用尚不清楚.
- 了解OTM反应的年龄相关差异是改善正牙治疗的关键.
研究的目的:
- 在OTM过程中调查线粒细胞在与年龄相关的骨重塑中的作用.
- 在机械应力下探索线粒细胞衰减对牙周带干细胞 (PDLSCs) 的影响.
- 确定在不同年龄组中增强OTM的潜在治疗目标.
主要方法:
- 在青少年和成年大鼠中建立了OTM模型.
- 在压缩负荷下使用了来自青少年和成人捐赠者的PDLSCs的体外研究.
- 评估了线粒的标记物 (PINK1,帕金),骨质细胞/骨质细胞活动,线粒体功能和RANKL/OPG比率.
- 采用siRNA来抑制线粒和Urolithin A/Mdivi-1来调节线粒在体内和体外.
主要成果:
- 根据年龄而言,正统牙强力增加了髓蛋白 (PINK1,帕金) 和骨质细胞/骨质细胞活性.
- 成年PDLSCs与青少年PDLSCs相比,显示了线粒体缩,线粒体功能受损以及较低的RANKL/OPG比率.
- 抑制线粒细胞衰变降低了线粒体功能和RANKL/OPG比率.
- 乌罗立A加速了OTM和骨重塑,而Mdivi-1抑制了这些过程.
结论:
- 在PDLSC中,强力刺激的线粒会在OTM过程中促进气膜骨重塑.
- 与年龄相关的线粒细胞衰减会对PDLSC对机械刺激的反应产生负面影响.
- 向线粒菌为优化不同年龄组的正牙治疗结果提供了一种新的治疗策略.
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