β-阻断剂驱动在纯粹和复杂的生物系统中多药耐药性基因的结合转移
Qian Wu1, Ge-Ge Wu1, Kai-Nan Pan2
1Laboratory of Water Pollution Remediation, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Journal of hazardous materials
|August 3, 2024
概括
非抗生素药物,如美托普罗罗尔可以增加细菌之间的抗生素耐药性基因 (ARG) 转移. 这通过诸如细胞透率增加和小胞形成等机制发生,造成健康风险.
科学领域:
- 环境微生物学环境微生物学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 广泛使用非抗生素药物与抗生素耐药性基因 (ARG) 的传播有关.
- 非抗生素药物对携带ARG的宽宿主等离子体在复杂环境中的转移的影响尚不清楚.
研究的目的:
- 研究梅托醇对ARG转移频率和宿主范围的影响.
- 阐明在各种细菌系统中由甲醇诱导的ARG转移的潜在机制.
主要方法:
- 结合性转移实验在内基和内基纯种植中.
- 细菌反应的分析,包括氧化应激,细胞膜透性,SOS反应和 pili 形成.
- 在一个复杂的anammox微生物组系统的调查.
- 对ARG和移动遗传元素的量化.
主要成果:
- 在环境度下,美托普罗罗尔显著促进了ARG的内基因和跨基因结合转移.
- 甲醇诱导了氧化应激,增加了细胞膜的透性,触发了细菌的SOS反应,并增强了 pili 的形成.
- 通过电子运输链激活,与结合相关的基因和ATP产量的增加促进了基因转移.
- 梅托普罗罗尔丰富了阿纳莫克斯系统中的ARG和移动遗传元素,促进了高结合转移频率.
结论:
- 梅托普罗罗尔通过促进细菌结合和促进转移到机会性病原体来增强ARG的传播.
- 非抗生素药物是抗生素耐药性的重要,被低估的驱动因素.
- 调查结果强调了与非抗生素制药污染相关的公共卫生和生态风险.
相关概念视频
00:57Antibiotic Selection
52.7K
Overview
52.7K
01:27Combined Effects of Drugs: Synergism
3.8K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
Such synergistic combinations...
3.8K
01:16Drug Biotransformation: Overview
2.4K
Pharmaceutical substances known as xenobiotics are predominantly lipophilic and nonionized. This enables them to permeate lipid bilayers, such as cell membranes, and interact with intracellular target receptors. Lipophilic drugs have an advantage in crossing biological barriers and reaching their intended sites of action. However, lipophilic drugs often have a restricted capacity for renal expulsion or elimination from the body. When these drugs enter the kidneys and undergo glomerular...
2.4K
01:27Nonlinear Pharmacokinetics: Role of Transporters
46
A drug's nonlinear kinetics can be influenced by a diverse range of transporter proteins that serve as crucial players in drug distribution. These transporters, found within cells, can enhance or reduce local drug concentrations by facilitating the influx or efflux of drugs. For instance, the expression of xenobiotic transporters can be influenced by factors such as age and gender, potentially impacting the linearity of drug response.
Polymorphisms occurring in drug transporters can alter...
Polymorphisms occurring in drug transporters can alter...
46
02:18Types of Genetic Transfer Between Organisms
27.2K
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
27.2K
02:13Overview of Transposition and Recombination
15.4K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.4K