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在便中检测胃肠道寄生虫的多重PCR:与直接显微镜和简单PCR进行基准测试

Puja Garg¹, Sadhna Lal Bhasin², Pankaj Malhotra³

¹Department of Medical Parasitology PGIMER, Chandigarh, 160012, India.

Diagnostic microbiology and infectious disease

|August 3, 2024

概括

一种新的多重PCR测试可以在便样本中准确检测多种肠道寄生虫,包括Cryptosporidium,Entamoeba histolytica和Giardia lamblia. 这种快速,具有成本效益的方法可以改善共感染的诊断,特别是在资源有限的环境中.

科学领域:

分子生物学分子生物学
寄生虫学的寄生虫学
传染性疾病传染性疾病

背景情况:

由原生动物引起的胃肠道感染很常见,特别是在资源有限的环境中.
同时感染多种寄生虫可能会使诊断和治疗复杂化.
现有的诊断方法,如显微镜,在灵敏度和特异性方面都有局限性.

研究的目的:

开发和验证用于同时检测Cryptosporidium spp.,Entamoeba histolytica和Giardia lamblia的多重常规PCR试验.
为流行原生动物共感染提供快速,经济有效和敏感的诊断工具.
在资源有限的环境中提高诊断准确性和效率.

主要方法:

从患有胃肠道症状的患者收集了便样本.
样品使用湿,和多重常规PCR进行处理.
科恩的kappa统计分析被用来评估方法之间的一致性.

主要成果:

在240名患者中,显微镜检测到28.75%的肠道原生虫.
多复合PCR显示100%与单复合PCR一致,检测到27.9%的阳性,通过测序证实.
在移植和免疫受损患者中发现了最高的寄生虫阳性,显微镜和分子方法之间有很好的一致性.

结论:

与显微镜相比,多重复合常规PCR提供了更高的灵敏度和特异性.
该试验能够从单个便样本中快速,经济有效地诊断多种寄生虫.
采用这种多重PCR可以显著提高常规诊断中的寄生虫感染管理.

关键词:

诊断诊断诊断诊断诊断肠道原生动物肠道原生动物显微镜的使用方法多重复合的传统PCR.资源有限的设置设置.

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