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酸核酸探针辅助纸质电化学生物传感器用于多重检测呼吸道病毒

Atchara Lomae¹, Kanyapat Teekayupak², Pattarachaya Preechakasedkit¹

¹Metallurgy and Materials Science Research Institute, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.

|August 3, 2024

概括

一种新的基于纸张的电化学分析装置 (ePAD) 能够快速,无标签地检测SARS-CoV-2,H1N1和RSV. 这种多重点检测试验为诊断这些呼吸道病毒感染提供了高灵敏度和特异性.

科学领域:

生物医学工程生物医学工程
传染病诊断传染病诊断传染病诊断
生物传感器技术技术

背景情况:

类似的SARS-CoV-2,H1N1和RSV的症状使诊断和管理复杂化.
多重复合点检测对迅速和有效的疾病控制至关重要.
目前的诊断方法可能缺乏广泛查所需的速度和多重复合能力.

研究的目的:

开发一种新的基于纸张的电化学分析装置 (ePAD),用于同时,无标签检测SARS-CoV-2,H1N1和RSV.
评估ePAD平台的敏感性,特异性和临床适用性.
为常见的呼吸道病毒感染提供快速准确的诊断工具.

主要方法:

开发一个ePAD平台,使用固定式pyrrolidinyl核酸探针.
基于探针和病毒核酸点之间的杂交变化进行电化学检测.
验证使用42个鼻拭片样本,并与逆转录聚合酶链反应 (RT-PCR) 进行比较.

主要成果:

该ePAD表现出高灵敏度与低检测极限 (SARS-CoV-2的0.12 pM,H1N1的0.35 pM,RSV的0.36 pM).
不同的病毒标之间没有观察到交叉反应.
临床验证显示与RT-PCR非常一致,达到100%的灵敏度和≥97%的特异性.

结论:

开发的ePAD平台是多重检测,无标签和无放大检测SARS-CoV-2,H1N1和RSV的一个有前途的工具.
该设备提供了高精度和高效率的查和诊断这些呼吸道病毒感染在 point-of-care.
这项技术有可能显著改善呼吸道传染病的管理.

关键词:

电压测量是指对电压的测量.电化学检测电化学检测没有标签的检测检测.多重复合检测检测多重复合检测基于纸质的分析装置.抽样样本从抽样样本中取出.

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