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使用竹纤维素支架进行碳酸酶封装,以有效捕获和转化CO2

Xiaoqiang Wang¹, Menghan Li¹, Zhiyuan Liu¹

¹State Key Laboratory of Heavy Oil Processing & College of Chemistry and Chemical Engineering, China University of Petroleum (East China), 66 West Changjiang Road, Qingdao, Shandong 266580, China.

International journal of biological macromolecules

|August 3, 2024

概括

使用竹纤维素的酶固定增强了碳酸 (CA) 的稳定性和可重复使用性,以有效捕获碳. 这种可持续的方法提高了CA在二氧化碳水合和微藻种植方面的性能.

科学领域:

生物催化剂是一种生物催化剂.
生物材料是一种生物材料.
环境科学环境科学

背景情况:

二氧化碳无水酶 (CA) 对于二氧化碳水合至关重要,这是碳捕获和利用的关键过程.
酶固定对于提高CA的稳定性和可重复使用性在工业环境中至关重要.
开发可持续和具有成本效益的固定化策略对于实际应用至关重要.

研究的目的:

在可再生竹纤维素支架上固定碳酸 anhydrase (CA).
评估固定CA的增强的催化性能,稳定性和可重复使用性.
评估固定CA对微藻生物质生产和生物分子合成的影响.

主要方法:

脱的竹纤维素通过氧化诱导的纤维素甲基化功能化.
碳酸无水酶 (CA) 通过使用Schiff基链接对改性纤维素支架进行了固定.
用p-NPA水解和CO2水化模型评估了催化活性.
在温度和pH值变化以及微藻培养下测试了稳定性和可重复使用性.

主要成果:

固定CA表现出最佳温度的增加 (约. 45°C) 和pH值 (大约. 9.0) 与自由 CA 相比.
CA活动保留率超过60%,更大的脚手架尺寸显示出更好的性能.
固定CA在5个周期后保持了80%以上的活性,并在恶劣条件下表现出高稳定性.
微藻生物质的产量增加了~16%,通过使用固定CA的增强生物分子合成.

结论:

在竹纤维素上轻松和绿色地固定CA,提高了酶的稳定性,可重复使用性和催化效率.
开发的固定式AC显示了二氧化碳转化和利用技术的巨大潜力.
竹纤维素作为一个有希望的可持续支架,用于生物技术应用中的酶固定.

关键词:

碳酸无水酶是一种碳酸无水酶.脱的竹子脚手架被拆除了.酶不移动化酶不移动化

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