谷氨杂交的多 (β-氨基) - - 塑纳米颗粒用于增强基因传递和生物安全性
Songwei Tan1, Caiyan Yuan2, Yuhe Zhu1
1School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Journal of advanced research
|August 3, 2024
概括
谷氨 (GSH) 通过减少反应性氧物种 (ROS) 来增强多 (β-氨基) (PBAE) 基因传递载体,改善基因转移效率和生物安全性,在体外和体内.
科学领域:
- 生物技术是生物技术.
- 基因治疗 基因治疗
- 纳米医学是一种纳米医学.
背景情况:
- 克里斯普尔/卡斯9技术推进了基因治疗,基因载体至关重要.
- 多 (β-氨基乙烯) (PBAE) 非病毒载体可以引起细胞毒性,原因是活性氧物种 (ROS) 的升高,影响基因转移疗效.
研究的目的:
- 开发一种提高PBAE载体的基因传递能力和生物安全性的策略.
- 为了减轻与PBAE载体相关的ROS诱导的细胞毒性.
主要方法:
- 准备了一种由PBAE-等离子体纳米颗粒 (NP) 与谷氨 (GSH) 组成的混合系统.
- 在体外和体内表征包括流细胞计,RNA-seq,PCR,桑格测序,成像和组织学.
主要成果:
- 与无GSH的NP相比,GSH-PBAE等离子体NP提高了基因传染和编辑效率10-100%.
- GSH-PBAE-等离子体NP减少了25-40%的细胞内ROS,最大限度地减少了基因表达障碍,并改善了细胞活力.
- 在体内研究表明,在EBV感染的小鼠模型中,优越的安全性和瘤抑制.
结论:
- GSH-PBAE混合系统提供了一种简单而有效的策略,以改善非病毒载体基因传递.
- 预计这种方法将促进基于PBAE的基因治疗的临床应用.
关键词:
基因输送 基因输送 基因输送葡萄糖氨酸 (Glutathione) 是一种纳米颗粒是如何形成的聚 (β-氨基乙烯) 乙烯 (β-氨基乙烯) 是一种聚 (β-氨基乙烯) 乙烯.这就是ROSOS ROS.更多相关视频
08:51Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
15.9K
07:40Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
Published on: July 31, 2015
8.7K
相关概念视频
01:09Recombinant DNA
Overview
00:53Transgenic Organisms
Overview
01:59Plant Breeding and Biotechnology
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
00:53Transgenic Organisms
Overview
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...