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氧化的信号影响了类似恐慌的防御行为和在前临床大脑皮层的防御性反感受神经调节
Renato Leonardo de Freitas1, Renata Moreira Acunha2, Fernando René Bendaña-Córdoba2
1Laboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo 14049-900, Brazil; Laboratory of Neurosciences of Pain & Emotions and Multi-User Centre of Neuroelectrophysiology, Department of Surgery and Anatomy, FMRP-USP, Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo 14049-900, Brazil; Institute of Neuroscience and Behaviour (INeC) Ophidiarium, Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo 14040-901, Brazil; Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", Naples 80138, Italy; Institute of Natural Sciences, Federal University of Alfenas (UNIFAL-MG), Str. Gabriel Monteiro da Silva, 700, Alfenas, 37130-000 Minas Gerais (MG), Brazil.
在前皮质 (PrL) 中的氧化 (NO) 调节了恐慌和恐惧反应. 在PrL中抑制或清理NO可以减少防御行为和在恐惧期间缓解疼痛.
科学领域:
- 神经科学是一个神经科学.
- 行为神经科学 行为神经科学
- 神经药理学神经药理学
背景情况:
- 中间前额皮层 (mPFC) 的前额皮层 (PrL) 在恐慌反应中起着至关重要的作用.
- 了解恐慌和防御行为背后的神经化学机制是必不可少的.
研究的目的:
- 为了调查氧化 (NO) 在PrL皮层中信号传递在防御行为和反感受调节中的作用.
- 为了确定NO是否在与恐慌相关的情绪反应中起到调解作用.
主要方法:
- 在PrL皮层中微注射Nω-propyl-L-arginine (NPLA),一种氧化合成酶 (NOS) 抑制剂,或Carboxy-PTIO (c-PTIO),一种NO清理剂.
- 随后用比库库林刺激下丘脑以引起防御行为.
- 记录探索性行为,防御反应和反感觉.
- 在脑组织中对c-Fos蛋白进行免疫标记,以识别神经元激活模式.
主要成果:
- 在PrL中用NPLA抑制NOS可以减少类似恐慌的反应和恐惧诱导的反恐感.
- 在PrL中用c-PTIO清理NO,降低了逃跑行为和防御性反感受.
- 在逃避行为之后,在新皮层,边缘和中脑结构中观察到c-Fos蛋白表达.
结论:
- 在PrL/mPFC中,氧化的信号传递对于组织逃跑行为和防御性反受体是至关重要的,由中介性下丘脑核调节.
- 面向逃避行为涉及一个包括新皮层,边缘和中脑区域的网络.
- 这些发现强调了NO在恐慌类反应期间在PrL皮质中的作用,表明它充当逆行信使.
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