以生物信息学和实验验证为基础,确定TFRC作为肺动脉高血压的生物标志物
Chuang Yang1, Yi-Hang Liu1, Hai-Kuo Zheng2
1Department of cardiology, The second hospital of Jilin University, Changchun, China.
Respiratory research
|August 3, 2024
概括
这项研究确定了TFRC作为肺动脉高血压 (PAH) 的潜在生物标志物. 在PAH患者的肺组织和血清中增加的TFRC表达和一个大鼠模型表明它对这种危及生命的疾病的诊断价值.
科学领域:
- 心肺医学 心肺医学
- 基因组学就是基因组学.
- 生物标志物发现发现
背景情况:
- 肺动脉高血压 (PAH) 是一种严重的慢性疾病,生物标志物研究有限.
- 现有的研究缺乏对PAH生物标志物基因表达特征的全面分析.
研究的目的:
- 确定肺动脉高血压 (PAH) 的新型基因表达生物标志物.
- 在患者队列和动物模型中验证已识别的生物标志物的诊断潜力.
主要方法:
- 结合并分析了两个基因表达数据集 (GSE131793,GSE113439) 以确定差异表达基因 (DEG).
- 利用权重基因共同表达网络分析 (WGCNA) 和蛋白质与蛋白质相互作用 (PPI) 网络分析来识别枢纽基因.
- 在独立数据集 (GSE53408,GSE22356) 和动物模型中进行了功能丰富分析 (GO,DO,KEGG,GSEA) 和验证了基因表达.
主要成果:
- 确定了914个DEG,其中722个是上调和192个是下调.
- 根据WGCNA和PPI的分析,TFRC被认为是一个重要的枢纽基因.
- 在验证数据集中,TFRC显示出强大的诊断价值,并且在PAH患者血清和肺高血压大鼠模型中得到上调.
结论:
- 生物信息分析显示,人类PAH肺组织中的TFRC表达增加,与患者数据和动物模型一致.
- TFRC显示出作为诊断肺动脉高血压的有价值生物标志物的潜力.
相关概念视频
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
1.2K
Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
1.2K
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
765
Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
765
01:22Rheumatic Heart Disease II: Clinical Manifestations and Diagnostic Studies
1.3K
The key clinical manifestations of Rheumatic heart disease (RHD) include several distinct cardiac symptoms.Carditis, a hallmark of acute rheumatic fever, involves inflammation of the heart's endocardium, myocardium, and pericardium. Chronic RHD often results from recurrent episodes of carditis. Its symptoms include the following:Murmurs are caused by valvular damage, especially to the mitral and aortic valves. Mitral stenosis or regurgitation is common, with characteristic heart murmurs...
1.3K