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Updated: Jun 18, 2025

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多色双激光超分辨率结构化照明显微镜用于可视化活细胞中的多有机体

Xuejuan Hu^1,2, Yadan Tan^1,2, Yujie Huang³

¹College of Physics Science and Technology, Guangxi Normal University, Guilin, Guangxi, China.

Journal of biophotonics

|August 4, 2024

概括

一个新的双激光成像系统使得四色超高分辨率显微镜只使用两个激光. 这种强大的平台可以详细观察活细胞中的细胞结构和动态.

科学领域:

细胞和分子成像技术
生物光子学生物光子学
超高分辨率的显微镜

背景情况:

先进的成像技术对于理解复杂的细胞过程至关重要.
多色成像允许同时可视化多个细胞组件.
现有的方法通常需要多个激光源,限制灵活性和增加复杂性.

研究的目的:

开发和验证一种用于超高分辨率显微镜的新型双激光多色成像系统.
为了比较光谱净化算法的有效性,用于四色成像.
建立一个高分辨率活细胞成像的多功能平台.

主要方法:

实现一个带有多通道过轮的双激光成像系统.
对非负矩阵因子化 (NMF),RCAN和PICASSO光谱净化算法的评估.
获取和重建四色超高分辨率图像.
在不同细胞系 (AML12,SH-SY5Y,HT-22,巨细胞) 中对各种器官 (脂质滴,线粒体,溶解体,细胞核) 的成像.

主要成果:

通过仅使用两个激光源,成功实现了四色成像.
该系统显示了所有四个通道的高空间分辨率 (125-133 nm).
观察到细胞动态,包括染色质凝聚,核收缩,DNA碎片化和线粒体自,以应对氧化应激.
启用了有机体融合 (线粒体和 lysosomes) 的可视化.

结论:

开发的双激光多色成像系统对于高分辨率的活细胞研究是有效的.
该系统为动态有机体研究提供了一个强大的平台.
这项技术促进了对细胞过程和应激反应的先进研究.

关键词:

这是一个SIMSIMSIMSIM.线粒体中的线粒体.多色多色的颜色.原子核中的原子核.氧化应激是一种氧化应激.不混合的光谱.

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