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在秘鲁成年人尿样中检测出Mycobacterium tuberculosis的经DNA

Annelies W Mesman¹, Roger I Calderon², Laura Hauns³

¹Department of Global Health and Social Medicine, Harvard Medical School, 641 Huntington Ave, Boston, MA, 02115, USA.

Tuberculosis (Edinburgh, Scotland)

|August 4, 2024

概括

在尿液中检测出Mycobacterium tuberculosis的经DNA显示出诊断肺结核 (TB) 的前景,特别是在涂抹阳性病例中. 这种非侵入性方法提供了一个潜在的替代品,当唾液收集是具有挑战性的.

科学领域:

分子诊断学分子诊断学
传染病研究传染病研究.
结核病诊断方法结核病诊断方法

背景情况:

肺结核 (TB) 诊断通常需要唾液,这并不总是可以获得的.
结核菌菌 (Mtb) 尿液中的经DNA (trDNA) 呈现出一种非侵入性的诊断替代方案.
之前的研究报告了mtbtrDNA检测的可变灵敏度.

研究的目的:

评估尿液中mtbtrDNA检测的灵敏度和特异性,用于诊断肺结核.
探索影响尿样中Mtb trDNA检测的因素.

主要方法:

通过使用短片段实时PCR对75名成年肺结核培养确认患者的3个连续尿样进行分析.
评估与Mtb trDNA检测相关的宿主,尿液和采样因素.
使用来自10个健康对照的尿样进行特异性测试.

主要成果:

对mtb trDNA检测的个体级敏感度为73%,在调整的测试值后增加到88%.
在涂抹阳性结核病患者 (92%) 中,敏感性显著更高.
健康对照群的特异性为100%;检测和患者/样本特征之间没有发现关联.

结论:

尿液mtb trDNA检测显示出作为肺结核的敏感和特定诊断工具的潜力.
尿样采集的非侵入性比传统的基于唾液的方法具有优势.
进一步优化测试和采集程序可能会提高诊断效用.

关键词:

没有细胞的DNA.诊断诊断诊断诊断诊断诊断诊断诊断诊断结核病是一种疾病.

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