通过使用硫化物处理污泥来实现部分化:确定最佳条件,评估长期稳定性和探索微生物机制
Xuliang Zhuang1, Danhua Wang2, Cancan Jiang3
1Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.
Bioresource technology
|August 4, 2024
概括
这项研究引入了一种新的方法,用于在废水处理中使用硫化物控制微生物活动的部分化 (PN). 这种方法有效地去除氨,同时促进稳定的PN,增强微生物的多样性和功能.
科学领域:
- 环境微生物学 环境微生物学
- 废水处理工程 废水处理工程
- 生物技术是生物技术.
背景情况:
- 实现稳定的部分化 (PN) 对于在废水处理中有效去除至关重要.
- 控制微生物群落,特别是氨氧化细菌 (AOB) 和氧化细菌 (NOB),是维持PN的关键.
- 现有的方法经常面临稳定性和效率方面的挑战.
研究的目的:
- 开发一种创新策略,以在合成家用废水中实现稳定的PN.
- 用硫化物作为唯一的控制因子来选择性抑制NOB活动.
- 优化硫化物处理条件,评估其对微生物群落和去除性能的影响.
主要方法:
- 进行可控批量实验,以确定最佳的硫化物处理参数.
- 用响应表面分析来确定硫化物处理的最佳条件.
- 使用长期反应堆运行来评估PN过程的稳定性和效率.
- 进行了微生物社区分析,包括基因表达 (amoA, soxB) 和网络分析.
主要成果:
- 确定了硫化物治疗的最佳条件:硫化物90mg/L,pH7.5,每分钟100转,治疗时间12小时.
- 硫化物处理显著抑制了NOB活动,同时保持了大量的AOB活动.
- 长期使用的PN反应器实现了83.9%的稳定化物积累率,其氨去除效率为99.1%.
- 硫化物治疗增强了微生物群落的多样性和稳定性,与amoA和soxB基因表达相关.
结论:
- 硫化物是有效的唯一控制因子,用于在废水处理中实现稳定的PN.
- 优化的硫化物处理策略提高了去除效率和微生物群落稳定性.
- 关键的微生物种群,包括Nitrosomonas和Thauera,被确定为治疗系统中持久PN的关键.
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