胚胎性酒精暴露会改变成年斑马鱼的胆固醇神经传递和记忆
Bárbara Fiorentin Giordani Gamba1, Karolyne de Pieri Pickler2, Guilherme Lodetti2
1Translational Psychiatry Laboratory, Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, SC, Brazil; Department of Physiology and Behavior, Federal University of Rio Grande do Norte, Natal, RN 59072-970, Brazil; Graduate Program in Psychobiology, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN 59072-970, Brazil; Laboratory of Experimental Neuropsychobiology, Department of Biochemistry and Molecular Biology, Natural and Exact Sciences Center, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS 97105-900, Brazil; Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, 1000 Roraima Avenue, Santa Maria, RS 97105-900, Brazil.
胚胎暴露于酒精会导致胎儿酒精谱系障碍 (FASD),损害成人记忆力并影响胆固醇系统. 斑马鱼模型显示,在产前暴露于酒精后,持续的认知缺陷.
科学领域:
- 神经科学是一个神经科学.
- 发育生物学 发展生物学
- 毒理学 毒理学 毒理学
背景情况:
- 酒精是全球消费的成性物质,对健康有重大影响.
- 产前酒精暴露可能导致胎儿酒精谱系障碍 (FASD),影响胚胎发育,特别是中枢神经系统 (CNS).
- 由FASD引起的神经发育问题在成年时表现为持续的认知和行为障碍.
研究的目的:
- 在FASD的斑马鱼模型中研究酒精对胆固醇信号和记忆的有害影响.
- 评估胚胎乙醇暴露后的长期记忆缺陷.
- 探索胆固醇系统变化和FASD中的认知障碍之间的关系.
主要方法:
- 使用斑马鱼模型研究FASD.
- 胚胎暴露于0.5%和1%的度中的乙醇.
- 在成年斑马鱼中进行行为测试 (抑制性回避,新型物体识别) 和分析胆固醇系统标记物 (ChAT,ACHE活性和基因表达).
主要成果:
- 胚胎乙醇暴露导致成年斑马鱼的记忆力受损,这在两个行为测试中都很明显.
- 这两种乙醇度都在抑制性回避测试中扰乱了记忆获取.
- 1%的乙醇在物体识别测试中影响记忆,0.5%的乙醇降低了CHAT和ACHE活动,表明胆固醇系统参与.
结论:
- 斑马鱼FASD模型有效地复制了在出生前暴露于酒精的个体中观察到的记忆障碍.
- 胚胎性酒精暴露导致持久的认知缺陷,并影响成年斑马鱼的胆固醇系统.
- 胆固醇系统的变化可能导致FASD观察到的认知障碍.
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