新5Gc介导的高 afinity 相互作用是 CD22 cis-ligands 调节 B 细胞信号传递的必要
Chizuru Akatsu1, Yuko Naito-Matsui2, Hajjaj H M Abdu-Allah3
1Department of Immunology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
CD22 (也称为Siglec-2) 与低亲和度连接体的相互作用增强了B细胞受体信号传递,但减少了强力信号传递. 这表明,高亲和度的CD22连接体可能通过与外部连接体竞争结合来调节B细胞.
科学领域:
- 免疫学 免疫学 免疫学
- 葡萄糖生物学 葡萄糖生物学
- 细胞信号传递 细胞信号传递
背景情况:
- CD22 (Siglec-2) 是一种抑制性B细胞受体,可以识别α2,6酸.
- CD22与cis-和trans-ligands相互作用,调节BCR和强力信号传递.
- 鼠标CD22对Neu5Gc具有很高的亲和力,而人类CD22则结合了不同的高亲和力连接体.
研究的目的:
- 为了研究高与低亲和度CD22连接体如何调节B细胞信号传递.
- 了解CD22连接体亲和力在B细胞功能中的作用.
主要方法:
- 使用Cmah-/-小鼠缺乏Neu5Gc和野生型B细胞.
- 分析了B细胞信号通路,包括BCR结合诱导和增强信号.
- 对缺乏Neu5Gc.的B细胞进行表型分析.
主要成果:
- CD22与内源配体的相互作用增强了BCR信号传递,减少了野生型和Cmah-/- B细胞中的强力信号传递.
- Cmah-/- B细胞没有显示与增强信号相关的表型的变化.
- 低亲和度CD22的cis-ligand相互作用足以调节B细胞信号传递.
结论:
- 低亲和度CD22的cis-ligand相互作用足以调节B细胞信号传递.
- 高亲和度 CD22 连接体可能通过与外源性跨连接体竞争 CD22 结合来调节 B 细胞.
更多相关视频
05:24Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
Published on: February 21, 2021
04:47Measuring Transcellular Interactions through Protein Aggregation in a Heterologous Cell System
Published on: May 22, 2020
相关概念视频
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
Immunoglobulin-like Cell Adhesion Molecules
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Assembly of Signaling Complexes
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
