通过纤维细胞激活蛋白成像,通过纤维细胞激活蛋白对急性损伤后的profibrotic不适应性修复进行动态可视化
Jiawen Huang1, Shuang Cui2, Xiaohua Chi3
1Division of Nephrology, Nanfang Hospital, Southern Medical University, State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Nephrology, Guangdong Provincial Clinical Research Center for Kidney Disease, Guangzhou, China; Department of Nuclear Medicine, Nanfang Hospital, Southern Medical University, Guangdong Medical Products Administration Key Laboratory for Quality Control and Evaluation of Radiopharmaceuticals, Guangzhou, China.
Kidney international
|August 4, 2024
概括
纤维细胞激活蛋白 (FAP) 成像非侵入性追踪急性损伤 (AKI) 后的不适应性修复. 持续的FAP上调预测纤维化,为早期干预提供了一个新的工具.
科学领域:
- 生物医学成像学 生物医学成像学
- 脏病理生理学脏病理生理学
- 纤维化研究纤维化.
背景情况:
- 评估器官纤维化进展的非侵入性方法仍然是一个挑战.
- 纤维细胞激活蛋白 (FAP) 涉及到亲纤维细胞的过程.
- 急性损伤 (AKI) 可以通过不适应性修复导致慢性病 (CKD).
研究的目的:
- 评估纤维细胞激活蛋白 (FAP) 成像,以动态,非侵入性评估AKI后的不适应性修复.
- 为了将FAP表达和成像信号与纤维化发展相关联.
- 探索FAP成像在预测AKI后纤维化结局方面的潜力.
主要方法:
- 在AKI小鼠模型和人体活检中,使用单细胞RNA测序和免疫染来描述脏FAP表达模式.
- 使用FAP抑制剂标记物 ([18F]FAPI-42,[18F]FAPT) 的正子发射断层扫描 (PET) /CT成像来追踪FAP活体中的活性.
- 相关PET/CT成像摄取与组织纤维化评估在AKI后的各种时间点.
主要成果:
- 在AKI之后,纤维细胞中的FAP被特别上调,与纤维细胞激活和CKD进展相关.
- 在AKI模型中,持续的脏FAP上调与持续的纤维细胞激活和纤维结局有关.
- [18F]FAPT PET/CT吸收非侵入性反映了持续的脏FAP上调和与AKI后脏纤维化发展相关.
- 类似的FAP成像变化在人类AKI患者中观察到,CKD进展.
结论:
- 在AKI后,脏FAP持续上调与不适应性修复和纤维化结局有关.
- 特定于FAP的PET/CT成像提供了AKI后不适应性修复的动态可视化.
- 这种成像方法可以在临床相关的时间框架内预测纤维化.
相关概念视频
01:22Acute Kidney Injury I: Introduction
Introduction:Acute Kidney Injury (AKI) describes a swift decrease in kidney function occurring over hours to days, characterized by the kidneys' failure to remove waste products from the bloodstream. This leads to dangerous complications like metabolic acidosis, fluid overload, and electrolyte imbalances, such as hyperkalemia, which can cause life-threatening arrhythmias. AKI is common in both hospital and outpatient settings, often triggered by dehydration, sepsis, or exposure to nephrotoxic...
01:29Acute Kidney Injury II: Pathophysiology
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
01:30Acute Kidney Injury IV: Diagnostic Studies and Prevention
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...