退化的Bletilla条纹多糖的保护作用,防止UVB诱导的皮肤氧化应激
Haoying Chen1, Changhui Lin1, Yan Wu1
1Plant Fiber Material Science Research Center, State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, PR China.
International journal of biological macromolecules
|August 4, 2024
概括
降解的布莱蒂拉条纹多糖 (DBSPs) 显示出改善的抗氧化和抗光衰老作用. DBSP-5有效地对抗UVB诱导的皮肤损伤和黑色素的产生,这表明局部皮肤治疗的潜力.
科学领域:
- 生物化学 生化学
- 皮肤病学 皮肤病学
- 材料科学 是一种材料科学.
背景情况:
- 布莱蒂拉条纹多糖 (BSP) 具有抗氧化特性,但由于高分子量和结构,其生物可访问性较差.
- 紫外线辐射诱导皮肤光衰老和氧化应激,需要有效的保护剂.
研究的目的:
- 研究降解BSP (DBSPs) 对皮肤细胞中UVB诱导的氧化应激的抗光衰老和抗黑色素生成作用.
- 评估具有不同分子重量的DBSPs的结构-活性关系.
主要方法:
- 在的协助下提取BSP,然后进行受控的降解,以获得具有减少分子量 (153.94,66.96和15.54kDa) 的DBSP.
- 在体外评估抗氧化活性,细胞毒性 (HSF细胞),抗增殖作用 (B16F10细胞) 和防治UVB诱导的氧化应激.
- 测量反应性氧物种 (ROS),黑色素含量,铁酶活性和甲 (MDA) 水平.
主要成果:
- 降解降低了BSP的分子量,同时保留了核心结构特征,增强了体外抗氧化活性.
- DBSP对人类皮肤纤维细胞 (HSF) 细胞没有细胞毒性,但抑制了黑色素瘤 (B16F10) 细胞增殖.
- DBSPs保护HSF和B16F10细胞免受UVB诱导的氧化应激,减少ROS和黑色素的产生. DBSP-5在减少MDA和抑制黑色素生成方面特别有效.
结论:
- 退化的Bletilla条纹多糖 (DBSPs) 显示出增强的抗氧化和保护作用,防止UVB诱导的皮肤损伤.
- 具有15.54kDa分子量的DBSP-5显示出作为一种局部治疗剂的显著潜力,用于治疗与氧化应激和光衰老相关的皮肤疾病.
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