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Updated: Jun 18, 2025

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关于同血统和异血统猪之间的单核酸变体和替代多基解的综合分析

Yu Han¹, Weilong Tu², Yingying Zhang²

¹Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Institute of Animal Husbandry & Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

International journal of biological macromolecules

|August 4, 2024

概括

亲生繁殖增加了同卵性单核酸多态性 (SNP),改变了猪的基因表达和替代多基化 (APA) 位点选择. 这项研究揭示了与遗传机制和关键调节基因相关的组织特异性APA变化.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
动物遗传学动物遗传学

背景情况:

亲生繁殖导致 homozygous 单核酸多态 (SNPs),影响基因表达和表型.
替代多基化 (APA) 是一个关键的转录后调节机制,影响基因功能.

研究的目的:

调查同胞繁殖诱导的同卵性SNP对卢chuan猪的APA地点选择的影响.
为了阐明基因机制背后的繁殖相关的APA法规.

主要方法:

基因组重新排序以确定同卵性SNP在近亲生猪.
在多个组织 (腿部肌肉,淋巴结,肝脏) 中进行3'mRNA测序 (mRNA-seq),以分析APA事件.
识别和分析多基化信号 (PAS) 的SNP.

主要成果:

亲生繁殖显著增加了猪基因组中的同卵性SNP积累.
特定组织的APA使用模式在内生和外生猪之间有所不同,与不同的生物过程有关.
鉴定了关键基因 (PUM1,SCARF1,RIPOR2,C1D,LRRK2) 参与APA调节,受PAS SNP在近亲繁殖中的影响.

结论:

由近亲繁殖产生的基因组同胞性对APA法规产生了深远的影响.
这项研究提供了关于近亲繁殖对基因表达的遗传特征和生物后果的宝贵见解.
这些发现为了解畜牧业和其他物种内生繁殖的影响提供了资源.

关键词:

同性异质性是一种同性异质性.免疫反应的免疫反应.这是一种内生繁殖.鲁川猪是什么意思? 鲁川猪是什么意思?肌肉的发展肌肉的发展聚亚脱化信号的信号是多重的.

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