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通过使用ISDra2-TnpB系统的炎症调节序列增强Lysozyme表达的抗乳腺炎基因编辑乳山羊的生成

Rui Feng¹, Jianglin Zhao¹, Qian Zhang¹

¹Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest Agriculture & Forestry University, Yangling, Shaanxi, 712100, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|August 5, 2024

概括

基因编辑通过增强酶 (LYZ) 基因表达产生了抵抗乳腺炎的山羊. 这种创新方法提高了疾病抵抗力,并减少了奶牛养殖业的经济损失.

科学领域:

动物遗传学和动物繁殖
分子生物学分子生物学
疾病耐药机制疾病耐药机制

背景情况:

乳腺炎是奶牛养殖业的一个主要经济挑战.
基因编辑为动物育种提供了精确的基因组操纵.
发展抗疾病的牲畜对于可持续农业至关重要.

研究的目的:

开发基因编辑乳山羊,增强乳腺炎耐药性.
使用ISDra2-TnpB系统进行向基因整合.
为了研究增强酶 (LYZ) 基因表达的疗效.

主要方法:

在乳牛山羊中使用ISDra2-TnpB基因编辑系统.
在LYZ基因促进器中整合了先天性炎症调节序列 (IRS).
诱导乳腺感染大肠杆菌 (大肠杆菌) 以评估耐药性.

主要成果:

经过基因编辑的山羊在受到大肠杆菌感染时LYZ表达增加.
观察到增强的乳腺炎耐药性,减少了PANoptosis激活,并减轻了血乳屏障损伤.
LYZ表达是针对大肠杆菌感染的特异性,表明有针对性的反应.

结论:

该研究提出了一种针对乳腺炎耐药动物的新型基因编辑策略.
实现了外源性无基因表达和疾病特异性.
这种方法为使用先进的基因编辑技术开发抗病牲畜提供了蓝图.

关键词:

在ISDra2-TnpB系统中.基因编辑的育种策略有山羊,有山羊.乳腺炎是一种乳腺炎.

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