在高葡萄糖环境下,肌细胞细胞和巨细胞之间的相互作用导致炎症反应和胰岛素敏感性受损
Wei Luo1, Yue Zhou2, Li-Ying Wang2
1Department of Sports and Health Sciences, Nanjing Sport Institute, Nanjing 210014, Jiangsu Province, China.
World journal of diabetes
|August 5, 2024
概括
高葡萄糖环境促进巨细胞的透和极化,导致骨肌肉炎症和胰岛素抵抗. 向巨细胞可能为肌肉缩和IR提供治疗策略.
科学领域:
- 骨肌肉生理学 骨肌肉生理学
- 免疫学 免疫学 免疫学
- 代谢障碍 代谢障碍 代谢障碍
背景情况:
- 骨肌肉对葡萄糖吸收至关重要,也是胰岛素抵抗的主要部位.
- 巨细胞与骨肌肉的相互作用会影响炎症和再生.
- 巨细胞透和极化在高葡萄糖 (HG) 诱导的骨肌肉IR中的作用仍然不清楚.
研究的目的:
- 在高葡萄糖条件下研究肌细胞和巨细胞之间的相互作用.
- 评估这些相互作用对骨肌肉炎症和胰岛素耐药性的影响.
主要方法:
- 在体内分析红外线小鼠骨肌中的巨细胞两极分化,使用免疫组织化学.
- 在HG条件下开发了肌肉细胞和巨细胞的体外共同培养系统.
- 使用CCK-8,流细胞计,ELISA,免疫光和2-NBDG等测试来评估细胞和分子变化.
主要成果:
- 高葡萄糖诱导的促炎性巨细胞极化 (F4/80+CD86+CD206-) 和肌纤维尺寸的减少在体内.
- 在体外,HG增加了促炎性巨细胞和细胞因子 (TNFα,IL-1β,IL-6),同时降低了抗炎性标志物.
- 在共同培养的肌细胞体中,HG 损害了肌体发生 (肌管区域,E-MHC) 和胰岛素敏感性 (GLUT4,葡萄糖吸收).
结论:
- 在高葡萄糖条件下,肌细胞细胞和巨细胞之间的相互作用有助于骨肌肉炎症和胰岛素抵抗.
- 巨细胞在骨肌肉的发展中发挥着重要作用.
- 巨体代表了骨肌缩和IR的潜在治疗标.
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