对齐的生物电子聚烯/原结构用于外围神经接口
Ryan P Trueman1, Owein Guillemot-Legris2, Henry T Lancashire3
1UCL Centre for Nerve Engineering, University College London, London WC1N 1AX, UK; Department of Pharmacology, UCL School of Pharmacy, University College London, London WC1N 1AX, UK.
Advanced engineering materials
|August 5, 2024
概括
这项研究开发了对齐的生物电子支架,使用原蛋白水凝中的聚烯纳米颗粒来弥合神经间隙. 这些支架的电刺激显著增强了受损神经组织中神经细胞的生长.
科学领域:
- 生物材料科学 生物材料科学
- 神经科学是一个神经科学.
- 组织工程是组织工程.
背景情况:
- 神经损伤在临床治疗中带来了重大挑战.
- 目前神经修复的策略通常涉及弥合神经间隙,这仍然是一个复杂的问题.
- 生物电子材料为增强神经再生和接口提供了潜力.
研究的目的:
- 创建一个对齐的生物电子结构,以弥合神经间隙.
- 研究导电性聚烯 (PPy) - 原水凝支架在支持神经生长中的有效性.
- 评估电刺激对这些支架内的神经元再生的影响.
主要方法:
- 通过将多聚烯纳米颗粒嵌入到对齐的原体水凝中,制造3D生物电子支架.
- 用来自背部根的原始老鼠神经元播种支架.
- 在体外评估神经元外生长和长度,以响应脚手架组成和电刺激.
主要成果:
- 与对照原体结构相比,PPy载荷的原体结构显示神经元长度增加了1.7倍.
- 对PPy-原结构的电刺激导致神经元长度额外增加1.8倍.
- 通过生物电子支架证明了增强的神经元支持和增长促进.
结论:
- 由PPy-原复合材料组成的对齐生物电子支架显示了神经组织工程的巨大潜力.
- 电刺激进一步增强了这些生物电子结构的再生能力.
- 这项工作为开发用于神经接口和神经修复应用的先进生物电子材料提供了基础.
相关概念视频
01:21G-protein Coupled Receptors
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
02:52Stereoisomerism
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
01:26Protein Modifications in the RER
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
01:26Chemical Synapses
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
01:30Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
01:26Chemical Synapses
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...