相关实验视频

Updated: Aug 16, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes
10:05

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

Published on: August 13, 2012

11.3K

使用共聚焦显微镜鉴定PDZ结合缺陷Cx36突变体的ER保留缺陷的特征

Stephan Tetenborg1, Elizabeth Martinez-Soler1, John O Brien1

  • 1College of Optometry, University of Houston, Houston, TX, USA.

Bio-protocol
|August 5, 2024
PubMed
概括

研究人员开发了一个共聚焦显微镜协议,以识别Cx36间隙结蛋白中的ER保留缺陷. 这种方法可视化了ER衍生的"旋风",揭示了转染细胞中的蛋白质运输问题.

关键词:
连接 36 个 连接 36保持ER的保留时间在HEK293T细胞中.这是一个PDZ.走私和贩运的人口贩运

更多相关视频

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy11:03

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Published on: December 8, 2021

3.6K
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.2K

相关概念视频

Protein Modifications in the RER01:26

Protein Modifications in the RER

Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.