关于从立陶宛发酵食品中分离的Lactiplantibacillus plantarum 33C的基因组序列数据草案
Ashwinipriyadarshini Megur1, Eglė Lastauskienė2, Aurelijus Burokas1
1Department of Biological Models, Institute of Biochemistry, Life Sciences Center, Vilnius University, Sauletekio Ave. 7, LT, Vilnius 10257, Lithuania.
Data in brief
|August 5, 2024
概括
这项研究测序了来自立陶宛发酵桃番茄的Lactobacillus菌株的基因组. 虽然没有发现毒性或抗菌素耐药性基因,但已确定一种可转移的林氏素耐药性等离子体.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 食品科学 食品科学 食品科学
背景情况:
- 乳酸菌种在发酵食品中至关重要.
- 了解它们的基因组有助于食品安全和生物技术.
- 发酵的立陶宛桃番茄是微生物多样性的独特来源.
研究的目的:
- 执行从传统的立陶宛发酵桃番茄中分离出来的Lactobacillus菌株的全基因组测序.
- 识别与新陈代谢,抗菌素耐药性和毒性因素相关的基因.
- 分析等离子体的存在及其潜在影响.
主要方法:
- 全基因组测序和组装.
- 使用COG注释进行比较基因组分析.
- 使用PlasmidFinder服务器进行等离子体识别.
主要成果:
- 该基因组草案包括55个contigs,总计3,326,119 bp,GC含量为44.3%.
- 确定的主要蛋白质功能与转录,氨基酸运输和碳水化合物代谢有关.
- 在细菌基因组本身中没有检测到固有的抗微生物耐药性或毒性基因.
- 确定了塑pR18,赋予林氏素耐药性和可转移性.
结论:
- 测序的Lactobacillus菌株拥有具有显著代谢潜力的基因组.
- 缺乏内在耐药性/病毒性基因表明,在食品应用中具有潜在的安全性.
- 确定了可转移的林氏素耐药性等离子体,强调了监测食物传播细菌中的等离子体动态的重要性.
- 这些基因组数据是对乳杆菌和相关基因的比较研究的宝贵资源.
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