瘤抗原特异性互白素-10产生T细胞响应在头部和部状细胞癌患者
Momoka Horikawa1, Kei Masuda2, Hideyuki Takahashi3
1Faculty of Science and Technology, Division of Molecular Science, Gunma University, Kiryu, Gunma 376-8515, Japan.
Oncology letters
|August 5, 2024
概括
在头癌患者中存在产生瘤抗原特异性Interleukin-10 (IL-10) 的T细胞. 淋巴细胞激活基因-3 (Lag-3) 在T细胞上的表达影响IL-10的产生,提供潜在的治疗点.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 翻译医学是一种翻译医学.
背景情况:
- 介素-10 (IL-10) 是一种细胞因子,在免疫平衡,瘤免疫学和免疫疗法中发挥着关键作用.
- 了解癌症中产生IL-10的T细胞的动态对于开发有效的免疫疗法至关重要.
研究的目的:
- 为了研究在头支状细胞癌 (HNSCC) 患者中循环瘤抗原特异性IL-10产生T细胞的存在.
- 确定影响这些IL-10产生T细胞的免疫动力学因素,包括免疫检查点分子.
主要方法:
- 来自HNSCC患者的外周血液单核细胞 (PBMC) 用瘤抗原进行刺激 (p53,MAGE-A4).
- 使用酶相关免疫吸收点检测 (ELISPOT) 测试评估了干扰素 (IFN) -γ/IL-10的产量.
- 流细胞计分析了T细胞上的免疫检查点分子表达 (例如,淋巴细胞激活基因-3 [Lag-3]).
主要成果:
- 在显著比例的HNSCC患者中检测到瘤抗原特异性IL-10产生T细胞.
- 对于p53特异性反应,产生IL-10的T细胞比产生IFN-γ的T细胞更为普遍.
- 较低的Lag-3在T细胞表达与p53-特定的IL-10产生相关;Lag-3阻断在一些患者中增强了IL-10反应.
结论:
- 在HNSCC患者中存在循环瘤抗原特异性IL-10产生T细胞.
- 拉格-3表达T细胞在调节IL-10产生T细胞方面发挥作用,这表明它是一个潜在的治疗点.
- 这些发现为IL-10和Lag-3在抗瘤免疫反应中的作用提供了新的见解.
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