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通过supragel介导的胰腺原生群和功能性葡萄糖反应的小岛类群的高效生成

Hongmeng Ma¹, Lilin Xu¹, Shengjie Wu¹

¹State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, and College of Life Sciences, Nankai University, Tianjin, 300071, China.

Bioactive materials

|August 5, 2024

概括

新型生物材料Supragel有效地促进人类多能干细胞 (hPSCs) 分化为分泌胰岛素的细胞. 这种超分子凝支持集群形成,并增强内皮分化,用于再生医学应用.

科学领域:

生物材料科学生物材料科学
干细胞生物学干细胞生物学
再生医学是一种再生医学.

背景情况:

合成水凝对于人类多能干细胞 (hPSC) 培养至关重要.
关于影响hPSC血统分化和集群形成的生化线索的研究有限.
超分子凝 (Supragel) 是一种超分子凝,其在定向差异化方面的潜力正在被探索.

研究的目的:

调查Supragel在促进hPSCs岛屿类集群分化的有效性.
评估Supragel的刚性和生化成分在hPSC分化中的作用.
将Supragel基培养与Matrigel和悬浮培养等现有方法进行比较.

主要方法:

使用生物化的Supragel水凝形成.
在Supragel中培养hPSC,度为5mg/mL.
评估hPSC集群形成,刚度 (∼1.5 kPa),以及分化为内皮和胰岛素分泌细胞.
使用RGD和YIGSR序列通过整合素受体进行细胞相互作用.
与Matrigel和悬浮培养物的差异化效率进行比较.

主要成果:

由于其最佳的刚性,Supragel (5 mg/mL) 促进了自发的,均的hPSC集群形成.
苏普拉格尔促进了内皮和胰岛素分泌细胞的分化,通过RGD/YIGSR序列与hPSC相互作用.
与Matrigel和悬浮培养相比,观察到更高的分化效率,归因于集群大小和粘合点.
苏普拉格尔系统表现出对初始播种密度的耐受性,只需要静态培养条件.

结论:

苏普拉格尔是指导hPSC分化成小岛状细胞的有希望的生物材料.
液凝的机械和生化特性支持有效的干细胞分化和集群形成.
苏普拉格尔为产生胰岛素分泌细胞提供了一个强大的,不那么苛刻的培养系统,具有更广泛的生殖层分化的潜力.

关键词:

群岛群集是一个小群岛集群.机械硬度机械硬度机械硬度干细胞分化干细胞分化合成水凝是一种合成水凝.

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