在马来西亚的塞兰戈尔发现牛病毒性腹病毒的分子检测和基因定型
Nurulhidayah Khalid1, Siti Suri Arshad1, Nurhusien Yimer Degu2
1Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia.
Journal of advanced veterinary and animal research
|August 5, 2024
概括
在马来西亚的牛群中检测到牛病毒性腹 (BVD) 疾病病毒 (BVDV). 该研究确定了BVDV-1a,强调需要进行边境查,以防止BVDV亚基因型进一步进入.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
背景情况:
- 牛病毒性腹 (BVD) 是一种严重的牛疾病,由一种虫病毒引起.
- BVD表现为腹和免疫抑制,增加对其他疾病的易感性.
研究的目的:
- 在马来西亚塞兰戈尔的牛群中检测和分子表征牛病毒性腹病毒 (BVDV).
- 为了研究BVDV分离物的遗传多样性.
主要方法:
- 一项涉及253个牛血样本的横截面研究.
- 使用逆转录聚合酶链反应 (RT-PCR) 检测抗原.
- 用于基因分析的5'未翻译区域 (5'-UTR) 和E2基因的向放大.
主要成果:
- 在253个样本中,有一个对BVDV抗原呈阳性,其患病率为0.40%.
- 隔离的BVDV菌株 (UPM/MAL/BVDV/D17) 进行了分子特征.
- 该病毒根据E2基因序列被归类为亚基因型BVDV-1a.
结论:
- 马来西亚Selangor的牛群中BVDV正在积极传播.
- BVDV-1a的存在表明该地区可能存在多种不同的亚基因型.
- 实施对进口牛的边境查对于防止其他BVDV亚基因型进入马来西亚至关重要.
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