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在Xanthomonas perforans种群中多次获得XopJ2效应剂

Anuj Sharma^1,2, Fernanda Iruegas-Bocardo¹, Shaheen Bibi¹

¹Plant Pathology Department, University of Florida, Gainesville, FL 32611, U.S.A.

Molecular plant-microbe interactions : MPMI

|August 5, 2024

概括

一种新的Xanthomonas III型效应器XopJ2的变体,名为XopJ2b,已被识别和描述. 这种功能效应因子在Xanthomonas菌株中广泛存在,并有助于番茄的病原体适应性.

科学领域:

植物病理学植物病理学
微生物遗传学微生物遗传学
分子生物学分子生物学

背景情况:

第三类效应体 (T3E) 对Xanthomonas毒性至关重要,是作物耐药性的目标.
XopJ2 (AvrBsT) 是X. perforans中保存的YopJ家族T3E,它是番茄中细菌斑点疾病的原因.

研究的目的:

描述XopJ2 T3E的一个新发现的变种,XopJ2b.
研究Xanthomonas中XopJ2b的功能性保存和进化意义.

主要方法:

对XopJ2b序列和预测的3D结构的生物信息分析.
在胡和阿拉比多普西斯中进行功能性测试,以评估过敏反应 (HR) 诱导.
在各种Xanthomonas菌株中XopJ2b分布的基因组分析.

主要成果:

XopJ2b与XopJ2a共享保留的功能域和残留物,尽管其序列相同度为70%.
XopJ2b是一种功能性T3E,在植物细胞中触发HR,类似于XopJ2a.
XopJ2b广泛分布在多个Xanthomonas物种和地理区域,这表明横向的基因转移.

结论:

XopJ2b代表了Xanthomonas T3E曲目的显著扩展.
功能性保护强调了T3E的进化适应性.
广泛存在的XopJ2b强调了它对X. perforans在西红上的健康的重要性.

关键词:

在今年的春天里,克桑托蒙纳斯 (Xanthomonas) 是一种乙烯基转移酶的使用方法在这里,我们可以看到子的子.第三种类型的效应器.

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