来自Dolichos lablab种子的沙宁具有抗炎活性
Wei Zhang1, Fei Li1, Jiaming Cheng1
1State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, 301617 Tianjin, China.
Bioorganic chemistry
|August 5, 2024
概括
多利可斯 (Dolichos lablab) 种子含有具有抗炎功能的新型三烯酸盐. 这些化合物,包括21种新的氨酸,在细胞模型中有效降低了氧化的产生,这表明炎症性疾病的治疗潜力.
科学领域:
- 植物化学 植物化学
- 药理学 药理学是指药理学的学科.
- 传统中国医药 传统中国医药
背景情况:
- 杜利可斯 (Dolichos lablab) 种子被用于传统中医药,并以抗炎作用而闻名.
- 负责D. lablab种子抗炎活性的特定化合物在很大程度上仍未被描述.
- 三聚酸盐酸是D. lablab种子中发现的一类主要化合物.
研究的目的:
- 进行从D. lablab. 种子中提取的三类沙素的全面植物化学研究.
- 隔离和识别新的三烯酸盐,并评估它们的抗炎活性.
- 阐明已识别的抗炎化合物的作用机制.
主要方法:
- 使用染色学和光谱技术 (NMR,MS) 来分离和阐明三烯酸盐的结构.
- 在体外抗炎活性评估使用脂聚糖 (LPS) 刺激的RAW264.7巨细胞来测量氧化 (NO) 生产.
- 西方斑点分析用于调查关键炎症性细胞因子的表达水平,如瘤缩因子-α (TNF-α) 和介质素-6 (IL-6).
主要成果:
- 分离了21种新的三类沙素 (多利拉沙素A1-A4,B,C,D1-D3,E-M,N1,N2,O) 和13种已知的类似物.
- 化合物1-3,9,10,13-15,18,22,23和28-34在LPS诱导的RAW264.7细胞中显著抑制了氧化的产生,以剂量依赖的方式.
- 发现1,9,10,13,14和18的化合物通过降低TNF-α和IL-6的表达来抑制炎症反应.
结论:
- 这项研究成功地从具有显著抗炎潜力的D. lablab种子中鉴定出了新型三烯酸酸.
- 隔离的化合物,特别是1-3,9,10,13-15,18,22,23和28-34的萨波宁,通过抑制NO的产生,表现出抗炎活性.
- 抗炎作用通过对关键的炎症性细胞因子的降低调节来调节,突出了这些化合物的治疗前景.
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