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益生菌细菌吸附与 (II) 离子检测CRISPR/Cas12a系统相结合

Ying Yu¹, Yuan Zhang², Yining Zhao²

¹Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.

Biosensors & bioelectronics

|August 5, 2024

概括

益生菌细菌,如乳杆菌 (LGG) 有效地吸附离子 (Hg2+). 这种特性被用来开发一种基于CRISPR/Cas12a的灵敏光生物传感器,用于准确检测重金属.

科学领域:

环境科学环境科学
生物技术是生物技术.
分析化学分析化学

背景情况:

复杂的样本矩阵挑战了准确的重金属检测.
益生菌细菌显示出重金属吸附和矩阵干扰消除的潜力.

研究的目的:

开发一种敏感和特定的光生物传感器,用于检测离子 (Hg2+).
探索益生菌在生物传感器开发中的应用,用于重金属分析.

主要方法:

使用 Lactobacillus rhamnosus (LGG) 进行Hg2+吸附,使用弗洛伊德利希等温模型进行分析.
整合了CRISPR/Cas12a系统与LGG吸收的Hg2+来创建一个"Cas12a-MB"光生物传感器.
研究了将Hg2+吸附到LGG上抑制CRISPR/Cas12a激活的机制.

主要成果:

LGG表现出高的Hg2+吸附能力 (高达300μM),其中R2=0.9881.1.
"Cas12a-MB"生物传感器在90分钟内实现了Hg2+的0.44nM的低检测极限.
生物传感器显示,在LGG上吸附的Hg2+对CRISPR/Cas12a系统产生了特定的结合和抑制.

结论:

开发的"Cas12a-MB"生物传感器为Hg2+检测提供了一种新,环保和敏感的方法.
益生菌细菌提供了一个创新的功能单元,用于提高复杂矩阵中的生物传感器性能.
这项研究强调了益生菌在推进重金属检测技术方面的潜力.

关键词:

这就是CRISPR/Cas12a.生态友好型环保型 Hg(2+) 的检测能力.乳杆菌拉姆诺索斯 (LGG) 吸附性吸附微生物生物传感器

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相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...