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UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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使用反射光进行衰老检测.

Benjamin Dedic1, Leo Westerberg1, Andrea Mosqueda Solís1,2

  • 1Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.

Aging cell
|August 5, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了一种新的反射光方法,以准确量化衰老细胞,特别是在人类脂肪细胞中. 这种技术可以提高对与衰老相关的β-galactosidase (SABG) 活性的检测灵敏度,有助于对肥胖和2型糖尿病的研究.

关键词:
在SABG的基础上,一个X-gal.脂肪细胞 (adipocytes) 是一种脂肪细胞.这是一种β-galactosidase.布莱特菲尔德的明亮领域反射的光反射的光.衰老是一种老化.西部污染 (Western Blot) 是一种

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科学领域:

  • 细胞生物学 细胞生物学
  • 衰老研究研究 衰老研究
  • 代谢性疾病 代谢性疾病

背景情况:

  • 细胞衰老在发育,组织修复,癌症和衰老中起作用.
  • 衰老的增加与肥胖和2型糖尿病 (T2D) 有关.
  • 量化衰老细胞,特别是像人类脂肪细胞这样的大型原始细胞,是具有挑战性的.

研究的目的:

  • 介绍一种基于反射光的新方法,用于精确测量与衰老相关的β-galactosidase (SABG) 染色.
  • 为了能够精确量化各种细胞类型的衰老细胞,包括初级人类脂肪细胞.
  • 为研究脂肪细胞衰老及其与代谢障碍的联系提供了传统方法的优质替代方案.

主要方法:

  • 利用反射光显微镜与共聚焦成像检测SABG染色细胞中的X-gal晶体.
  • 综合反射光测量与免疫光用于全面的细胞状态分类.
  • 包含样本特定的pH值控制,用于强大的衰老评估.
  • 在初级人类和小鼠脂肪细胞中验证了该技术,并分化了3T3-L1细胞.

主要成果:

  • 与传统的亮场技术相比,在检测X-gal沉物方面获得了更高的灵敏度.
  • 捕获了各种X-gal染色模式,显示了更好的检测灵敏度.
  • 证明反射光分析提供了比西欧白斑更准确的SABG活性在成熟的人类脂肪细胞的量化.
  • 展示了该方法与免疫光和各种细胞类型的兼容性.

结论:

  • 新的反射光方法为量化细胞衰老提供了高度敏感和准确的方法,特别是在脂肪细胞中.
  • 这种技术有助于更深入地了解脂肪细胞衰老及其对肥胖和2型糖尿病的影响.
  • 反射光显微镜为衰老研究提供了一个比传统方法更全面,更敏感的工具.