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小麦根球微生物群的分类学和功能性变化是由基于伊米达的除草剂和基因改造引起的

Farman Ali¹, Zhaocheng Tang¹, Gangao Mo¹

¹Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

Environmental research

|August 5, 2024

概括

转基因小麦具有乙烯酸乳酸合成酶 (ALS) 突变,对土壤微生物群的影响最小. 然而,除草剂imazamox显著改变了微生物的多样性和丰富性,突出了除草剂使用的风险.

科学领域:

农业科学农业科学
微生物学微生物学
环境科学环境科学

背景情况:

转基因作物被广泛讨论,需要进行生物安全评估.
对非转基因转基因作物对土壤微生物的影响的研究是有限的.
了解除草剂对植物微生物群的影响对于环境风险评估至关重要.

研究的目的:

为了评估一种乙烯酸乳酸合成酶 (ALS) 突变小麦 (WK170B) 和 imazamox (IM) 除草剂对小麦根系生物群的影响.
评估非转基因转基因作物的生物安全性和相关的除草剂使用.
为了识别对IM除草剂暴露敏感的微生物种群.

主要方法:

小麦树根生物组的高通量测序.
在正常条件下对ALS突变种 (WK170B) 和对照种 (YN19B) 小麦进行比较分析.
小麦暴露于伊马扎莫克斯 (IM) 除草剂.
分类学,α和β多样性分析.
线性差异分析效果大小 (LEfSe),MetaStat和SIMPER分析微生物群落变化.
功能性基因分析.

主要成果:

突变ALS WK170B对根球微生物群的影响很小,蓝藻细菌的显着增加.
IM除草剂显著降低了特定细菌 (如Ralstonia pickettii) 的丰富性,并增加了微生物的丰富性和多样性.
包括Massilia,Limnobacter,Hydrogenophaga,Ralstonia,Nitrospira和Ramlibacter在内的微生物种类对IM暴露很脆弱.
参与细胞外基因与受体相互作用的基因在IM暴露后显著增加.

结论:

突变ALS的小麦对树根生物群的影响很小,这表明对细菌群体的潜在益处.
IM除草剂显著改变小麦树根生物组的组成和功能,表明潜在的环境风险.
确定了对IM除草剂敏感的关键微生物种群,为未来的风险评估提供了基础.

关键词:

艾尔斯突变小麦的小麦高通量测序的高通量测序基于伊米达佐林的除草剂微生物组是一个微生物组.抗压力抗压力抗压力

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