解冷化 (冷干燥) 人类尸体皮肤的生物物理和透性表征
Harini Priya Pa1, Pragathi S G1, Poornima K1
1Institute for Drug Delivery and Biomedical Research, Bangalore, India.
概括
化,一种冷干燥方法,为维护人体尸体皮肤进行体外透测试 (IVPT) 提供了经济有效的替代方案. 这项研究发现,冷干燥皮肤保持其屏障完整性和透性,与新鲜皮肤相比.
科学领域:
- 皮肤病学 皮肤病学
- 生物材料科学 生物材料科学
- 药理动力学 药理动力学
背景情况:
- 在体外浸透测试 (IVPT) 通常使用冷的人体尸体皮肤.
- 冷保存是昂贵的,繁的,并且可能会损害皮肤屏障的完整性.
- 长时间的冷和冷解周期会对皮肤结构产生负面影响.
研究的目的:
- 评估冷化 (冷干燥) 作为人类尸体皮肤的保存方法.
- 为了确定冷干燥对皮肤屏障特性和透性的影响.
- 评估冷解皮肤对IVPT应用的适用性.
主要方法:
- 人体尸体的皮肤样本经过冷化.
- 在对照和冷干燥皮肤上进行了形态测量分析和组织学研究.
- 测量了生物物理参数,包括跨皮体水蒸发 (TEWE) 和跨皮体电阻力 (TEER).
- 使用咖啡因和尼古丁作为模型透剂进行了体外透研究.
主要成果:
- 组织学检查显示,冷干燥后皮肤层组织或完整性没有显著改变.
- 在对照和冷干燥皮肤之间,在TEWE或TEER中没有发现显著差异.
- 水友性 (咖啡因) 和脂友性 (尼古丁) 模型化合物的透性在不同皮肤类型中保持一致.
结论:
- 解热化是人类尸体皮肤冷保存的可行替代方案.
- 冷干燥不会显著损害皮肤的屏障功能或透性.
- 软化皮肤适合用于局部产品的体外透性测试.
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