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一项全面的单细胞RNA转录组分析确定了老化骨肌肉中独特的SPP1+巨子组

Wen Bi¹, Mengyue Yang², Mengjia Shi³

¹Department of Sports Medicine, The Sixth Affiliated Hospital of Shenzhen University, Shenzhen Nanshan People's Hospital, Shenzhen, 518052, China.

Scientific reports

|August 5, 2024

概括

老化骨肌肉显示SPP1+巨细胞增加,与衰老和脂肪积累有关. 达沙替尼 + 奎尔塞丁的老化治疗减少了这些巨细胞,为与年龄相关的肌肉衰弱提供了潜在的治疗标.

科学领域:

细胞衰老细胞衰老
肌肉生物学肌肉生物学
免疫学免疫学免疫学

背景情况:

骨肌肉 (SkM) 衰老有助于与年龄相关的衰弱和残疾.
在SkM内的巨细胞 (Mac) 种群在衰老中发挥着不同的作用.
损坏和修复过程之间的不平衡与SkM功能下降有关.

研究的目的:

为了比较新陈代谢途径和细胞的生物功能在年轻和老老鼠的SkM.
在老年SkM中识别和描述特定的巨细胞子组.
研究向与衰老相关的巨细胞的治疗潜力.

主要方法:

综合单细胞转录组分析年轻和老老鼠的SkM.
巨细胞子组的识别和表征.
在小鼠四头肌中对SPP1+巨细胞比例的定量分析.
评估老年治疗药物组合 (达沙替尼 + 奎尔塞丁) 的影响.

主要成果:

在老老鼠的SkM中发现了一种表达高水平SPP1的独特的巨子组,表现出衰老和脂肪生成特征.
在老老鼠的四头肌中,SPP1+巨细胞的比例显著增加.
达沙替尼 + 奎尔塞丁的老年治疗组合显著降低了SPP1+巨细胞的比例.

结论:

SPP1+巨体代表着与SkM衰老和脂肪生成相关的独特细胞组成部分.
用达沙替尼 + 奎尔塞丁等老化剂向SPP1+巨细胞可能为SkM衰老提供了一种新的治疗策略.
这项研究强调了SPP1+巨细胞作为对抗与年龄相关的肌肉衰退的潜在老年治疗标.

关键词:

细胞衰老细胞衰老在SPP1+巨细胞中.年老的药物.单细胞测序是一种单细胞测序.骨架肌肉是一个骨肌肉.

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