相关实验视频
Updated: Jun 17, 2025

11:52
Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
Published on: April 23, 2016
8.4K
一角小麦中间体的进化是由两个LTR逆转移子的相互作用驱动的
Matthias Heuberger1, Dal-Hoe Koo2, Hanin Ibrahim Ahmed3,4
1Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
Mobile DNA
|August 5, 2024
概括
一角小麦的中间体利用两个逆转移子家族,RLG_Cereba和RLG_Quinta,进行功能. 这些元素与宿主建立了相互关系,动态地保持了中心分子的完整性.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 植物科学 植物科学
背景情况:
- 中心分子DNA序列在真核生物之间有很大差异,通常具有类似可转移元素 (TE) 的重复性DNA.
- 一角小麦的中间体单独包括RLG_Cereba和RLG_Quinta LTR反转移子,缺乏并列重复.
- 这些TE家族对中粒体功能的关系和功能贡献在很大程度上是未知的.
研究的目的:
- 为了研究RLG_Cereba和RLG_Quinta逆转移子家族在单角小麦中心体中的关系.
- 确定这些TE家族如何为中粒体功能和进化做出贡献.
主要方法:
- 预测GAG蛋白质的遗传学分析.
- 染色体免疫沉测序 (ChIP-seq) 用于识别TE的位置.
- 在活跃的中间体内分析TE群体.
主要成果:
- RLG_Cereba和RLG_Quinta的功能分别是自主和非自主合作伙伴.
- RLG_Cereba已经在单种植物中持续存在超过1亿年;RLG_Quinta在28-35万年前从中进化.
- RLG_Cereba整合酶的CR域向活跃的中间体,其中LTR与CENH3基因组变体结合,促进中间体完整性.
结论:
- RLG_Cereba促进了非自主RLG_Quinta的转让.
- 新的TE插入发生在功能性中间体中,通过整合酶CR域与CENH3.3结合.
- TE LTRs招募CENH3,建立一种相互相互作用,维持小麦中间体.
相关概念视频
LTR Retrotransposons
17.4K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.4K
Overview of Transposition and Recombination
15.4K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.4K
Non-LTR Retrotransposons
11.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K
Conservative Site-specific Recombination and Phase Variation
6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.0K
DNA-only Transposons
14.4K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.4K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K

