探索酵母生物多样性和工艺条件,以优化乙烯糖醇转化为甘油酸的过程
Vittorio Giorgio Senatore1, Riccardo Milanesi1, Fiorella Masotti1
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.
FEMS yeast research
|August 6, 2024
概括
酵母可以将塑料副产品乙烯糖醇 (EG) 转化为有价值的甘油酸 (GA). 这项研究优化了酵母菌株的高效GA生产,为塑料废物再循环铺平了道路.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 环境科学 环境科学
背景情况:
- 塑料废物,特别是聚乙烯四甲酸盐 (PET),由于回收率低,造成了重大的环境挑战.
- 乙烯糖醇 (EG) 是PET生物循环过程中释放的关键单体,在常见实验室酵母中具有有限的已确定的代谢途径.
- 目前的研究主要集中在EG的细菌代谢上,因此基于酵母的生物转化尚未得到充分研究.
研究的目的:
- 研究Saccharomyces cerevisiae和其他酵母物种消耗乙烯糖醇 (EG) 的能力.
- 确定能够有效地生产甘油酸 (GA) 的酵母菌株,作为EG的有价值的副产品.
- 优化生物转化过程,以增强GA生产,以实现潜在的塑料废弃物回收利用.
主要方法:
- 对十种常见的实验室酵母菌种进行EG消费和GA生产的选.
- 在Saccharomyces cerevisiae中使用实验设计方法优化EG到GA的两步生物转化过程.
- 生物反应器发酵精选的酵母菌株,包括Scheffersomyces stipitis,用于高度GA生产.
主要成果:
- 麦芽菌 (Saccharomyces cerevisiae) 显示出消耗EG和产生GA的能力,在优化条件下,GA的产量为4.51g/L.
- 查发现Scheffersomyces stipitis是一种优越的GA生产者,在单步生物工艺中达到23.79g/L.
- 实现了高的转化率 (94.25%) 和产量 (76.68%),证明了高效的EG到GA生物转化潜力.
结论:
- 酵母生物多样性为乙烯糖醇 (EG) 转化为甘油酸 (GA) 的生物转化提供了一个有前途的途径.
- Scheffersomyces stipitis对EG的工业规模GA生产具有显著的潜力,有助于塑料废弃物的利用.
- 这项研究为开发塑料衍生单体的可持续酵母基上循环利用策略奠定了基础.
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