不同的PFAS产生独特的转录基因变化,与斑马鱼的发育毒性有关
Yvonne Rericha1,2, Lindsey St Mary1,2, Lisa Truong1,2
1Environmental and Molecular Toxicology Department, College of Agricultural Sciences, Oregon State University, Corvallis, OR, United States.
Frontiers in toxicology
|August 6, 2024
概括
和多基基物质 (PFAS) 通过各种机制引起毒性. 这项研究使用斑马鱼将PFAS结构与基因表达变化联系起来,揭示了对大脑和神经系统发育的影响.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 分子生物学分子生物学
背景情况:
- 和多醇基物质 (PFAS) 是持久性环境污染物,毒性机制不明.
- 全面了解多种 PFAS 结构如何影响生物系统对于危险评估至关重要.
研究的目的:
- 为了研究斑马鱼对15种生物活性PFAS的转录反应.
- 为了将PFAS化学结构与观察到的毒性和基因表达模式相关联.
- 阐明PFAS发育性毒性背后的作用模式.
主要方法:
- 斑马鱼胚胎在受精后8小时暴露在15种优先级的PFAS中.
- 在受精后48小时进行RNA测序,以分析转录变化.
- 对精选化合物的内部PFAS度和时间转录组反应进行了测量.
主要成果:
- 暴露于PFAS诱导了各种不同表达的基因,主要影响大脑和神经系统的发育.
- 类似的PFAS功能头组与类似基因组的破坏有关.
- 与硫胺PFAS相比,硫酸PFAS与Nafion副产品2一样,显示出更高的身体负担和随着时间的推移增加的转录效应.
结论:
- 包括结构和功能组在内的PFAS化学特征影响毒性和转录破坏.
- 斑马鱼的转录组学提供了关于PFAS作用模式和发育毒性的见解.
- 调查结果有助于优先考虑PFAS进行进一步测试,并为危险评估提供信息.
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