Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Herniated Intervertebral Disc l: Introduction01:29

Herniated Intervertebral Disc l: Introduction

Intervertebral disc herniation refers to the displacement of the nucleus pulposus (the gel-like inner core of the disc) through a tear or weakened area in the annulus fibrosus (the outer fibrous ring). The displaced disc material extends beyond the normal boundaries of the disc space and may compress or irritate nearby spinal nerve roots or, less commonly, the spinal cord.Etiology and Risk FactorsHerniation commonly results from degeneration, in which aging reduces disc hydration and...
Degenerative Disc Disease I: Introduction01:27

Degenerative Disc Disease I: Introduction

Degenerative disc disease is a chronic condition in which intervertebral discs gradually lose structure and function. It is not infectious or autoimmune; rather, it results from age-related biochemical and mechanical changes, influenced by genetic, metabolic, and environmental factors.Structure and Function of DiscsThe spine contains 23 intervertebral discs that absorb load, distribute forces, maintain spacing, and allow flexibility. Each disc consists of a nucleus pulposus, a gel-like core...
Degenerative Disc Disease ll: Pathophysiology01:23

Degenerative Disc Disease ll: Pathophysiology

The symptoms of degenerative disc disease arise from a combination of mechanical compression, vascular compromise, and biochemical inflammation, which together disrupt nerve function and produce pain.Mechanical CompressionDisc degeneration reduces height and elasticity, predisposing to herniation of the nucleus pulposus, a major cause of radicular pain. Herniations may be protrusion (bulging with intact annulus), extrusion (nucleus extends beyond disc but remains connected), or sequestration...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A high throughput platform for measuring and predicting vitrification behavior in multicomponent aqueous solutions.

bioRxiv : the preprint server for biology·2026
Same author

A High-Throughput Platform for Measuring and Predicting Vitrification Behavior in Multicomponent Aqueous Solutions.

ACS applied materials & interfaces·2026
Same author

Water and cryoprotectant permeability of mature equine oocytes: experimental measurements and in silico predictions.

Scientific reports·2026
Same author

A Decade of Leadership and Impact: Celebrating 10 Years of the ORS Spine Section.

JOR spine·2026
Same author

Screening for cryoprotective agent toxicity and toxicity reduction in mixtures at subambient temperatures.

Cryobiology·2025
Same author

High-throughput evaluation of cryoprotective agents for mixture effects that reduce toxicity.

Cryobiology·2025

相关实验视频

Updated: Jun 25, 2026

Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
13:37

Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture

Published on: February 2, 2012

14.5K

冷保存完整的椎间盘,而不会影响生存能力.

Ward Shalash1, Ryan Forcier1, Adam Z Higgins1

  • 1School of Chemical, Biological and Environmental Engineering Oregon State University Corvallis Oregon USA.

JOR spine
|August 6, 2024
PubMed
概括

脊椎间盘 (IVD) 的冷保存现在可以通过一种新的方法加速冷保护剂 (CPA) 递送. 这种技术在冷储存后确保了高细胞活力,克服了用于移植和研究的组织保存的先前障碍.

科学领域:

  • 生物医学工程 生物医学工程
  • 再生医学是一种再生医学.
  • 组织工程是组织工程.

背景情况:

  • 像椎间盘 (IVD) 这样的组织的冷保存受到冷保护剂 (CPA) 的毒性和它们的传递速度缓慢的阻碍.
  • 器官移植和再生疗法研究目前的局限性部分是由于长时间保存组织和器官的挑战.
  • 人体IVD为治疗背痛和推进再生疗法提供了重大机会,但器官获取和运输的后勤问题阻碍了它们的使用.

研究的目的:

  • 为完整的椎间盘 (IVD) 开发和验证一种新的冷保存协议.
  • 显著加快冷保护剂 (CPA) 进入IVD的输送,以减轻毒性和细胞死亡.
  • 为了使IVD的长期存储具有保存的组织活力,用于潜在的移植和研究应用.

主要方法:

  • 在牛核细胞上测试了各种冷保护剂 (CPA) 溶液,以确定最少的细胞毒性选择.
  • 使用计算机断层扫描 (CT) 用CPA对比度增强成像,在动态负载和膨胀条件下,量化完整的牛IVD中的和时间.
  • 通过在溶解后在多个IVD区域评估细胞活力,验证了冷保存协议,包括一个星期的冷储存.

主要成果:

  • 使用二甲基硫氧化物和乙烯糖醇的冷保存介质在细胞毒性之前显示了超过7.5小时的安全性.
  • 动态加载和膨胀协议将IVD CPA和时间从3天以上减少到5小时以下,是20倍以上的改善.
关键词:
冷保存的方法盘子移植 盘子移植 盘子移植椎间盘之间的椎间盘.运输现象 运输现象

更多相关视频

An In Vitro Organ Culture Model of the Murine Intervertebral Disc
08:03

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Published on: April 11, 2017

9.5K
Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
06:22

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

2.1K

相关实验视频

Last Updated: Jun 25, 2026

Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
13:37

Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture

Published on: February 2, 2012

14.5K
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
08:03

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Published on: April 11, 2017

9.5K
Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
06:22

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

2.1K
  • 经过一周的冷保存后,所有测试的IVD区域都显示出85%的细胞活力,与新鲜,未冷的对照相比.

    • 结论:

    • 开发了一种新的方法,通过压缩后的胀,快速将CPA输送到完整的IVD中,解决了组织冷保存的关键挑战.
    • 这种加快的CPA透可以有效地冷保存IVD,而不会显著地降低细胞活力.
    • 优化的协议促进了IVD移植银行潜在的发展,并提高了它们在再生医学研究中的实用性.