冷保存完整的椎间盘,而不会影响生存能力
Ward Shalash1, Ryan Forcier1, Adam Z Higgins1
1School of Chemical, Biological and Environmental Engineering Oregon State University Corvallis Oregon USA.
JOR spine
|August 6, 2024
概括
脊椎间盘 (IVD) 的冷保存现在可以通过一种新的方法加速冷保护剂 (CPA) 递送. 这种技术在冷储存后确保了高细胞活力,克服了用于移植和研究的组织保存的先前障碍.
科学领域:
- 生物医学工程 生物医学工程
- 再生医学是一种再生医学.
- 组织工程是组织工程.
背景情况:
- 像椎间盘 (IVD) 这样的组织的冷保存受到冷保护剂 (CPA) 的毒性和它们的传递速度缓慢的阻碍.
- 器官移植和再生疗法研究目前的局限性部分是由于长时间保存组织和器官的挑战.
- 人体IVD为治疗背痛和推进再生疗法提供了重大机会,但器官获取和运输的后勤问题阻碍了它们的使用.
研究的目的:
- 为完整的椎间盘 (IVD) 开发和验证一种新的冷保存协议.
- 显著加快冷保护剂 (CPA) 进入IVD的输送,以减轻毒性和细胞死亡.
- 为了使IVD的长期存储具有保存的组织活力,用于潜在的移植和研究应用.
主要方法:
- 在牛核细胞上测试了各种冷保护剂 (CPA) 溶液,以确定最少的细胞毒性选择.
- 使用计算机断层扫描 (CT) 用CPA对比度增强成像,在动态负载和膨胀条件下,量化完整的牛IVD中的和时间.
- 通过在溶解后在多个IVD区域评估细胞活力,验证了冷保存协议,包括一个星期的冷储存.
主要成果:
- 使用二甲基硫氧化物和乙烯糖醇的冷保存介质在细胞毒性之前显示了超过7.5小时的安全性.
- 动态加载和膨胀协议将IVD CPA和时间从3天以上减少到5小时以下,是20倍以上的改善.
- 经过一周的冷保存后,所有测试的IVD区域都显示出85%的细胞活力,与新鲜,未冷的对照相比.
结论:
- 开发了一种新的方法,通过压缩后的胀,快速将CPA输送到完整的IVD中,解决了组织冷保存的关键挑战.
- 这种加快的CPA透可以有效地冷保存IVD,而不会显著地降低细胞活力.
- 优化的协议促进了IVD移植银行潜在的发展,并提高了它们在再生医学研究中的实用性.
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