Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Challenges and recommendations in establishing national human diversity genomic projects.

Nature methods·2026
Same author

Mendelian randomization studies in atopic dermatitis: causal insights across omics layers.

Frontiers in immunology·2026
Same author

Gene-sized editing for the therapy of genetic diseases.

Functional & integrative genomics·2026
Same author

AI-Based Prediction of Gene Expression in Single-Cell and Multiscale Genomics and Transcriptomics.

International journal of molecular sciences·2026
Same author

The <i>CTDP1</i> Founder Variant in CCFDN: Insights into Pathogenesis, Phenotypic Spectrum and Therapeutic Approaches.

International journal of molecular sciences·2026
Same author

Shedding light on DNA methylation and its clinical implications: the impact of long-read-based nanopore technology.

Epigenetics & chromatin·2024

相关实验视频

Updated: Jun 17, 2025

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
09:05

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Published on: May 2, 2025

612

多复合空间转录学方法和扩展显微镜的应用.

Andra Fortner1,2, Octavian Bucur2,3

  • 1Medical School, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany.

Frontiers in cell and developmental biology
|August 6, 2024
PubMed
概括

像ISS,ISH和NGS这样的空间转录学方法揭示了细胞组织. 将它们与扩展显微镜 (ExM) 结合起来,可以增强RNA检测,并推进生物和医学洞察力.

关键词:
过去的ST-ST.这就是ExSeq.渔业问题 渔业问题墨菲鱼是什么意思 墨菲鱼这就是Visium Visium.扩张显微镜扩张显微镜空间奥米克斯空间奥米克斯翻译学 翻译学 翻译学 翻译学

更多相关视频

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.5K

相关实验视频

Last Updated: Jun 17, 2025

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
09:05

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Published on: May 2, 2025

612
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

4.9K
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.5K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 空间转录学彻底改变了细胞组织研究.
  • 许多创新的转录组学方法已经出现,包括现场测序 (ISS),现场杂交 (ISH) 和基于下一代测序 (NGS) 的区域捕获.
  • 一个关键的挑战是由于光学拥挤而有限的RNA检测.

研究的目的:

  • 审查基于ISS,ISH和NGS的空间转录组协议.
  • 探索扩展显微镜 (ExM) 如何增强这些技术.
  • 突出转录学在生物学和医学上的更广泛影响.

主要方法:

  • 对空间转录学技术 (ISS,ISH,NGS) 的现有文献的审查.
  • 扩展显微镜 (ExM) 原理和应用的讨论.
  • 将ExM与空间转录组学协议集成在一起.

主要成果:

  • ExM克服了光学拥挤和衍射极限,改善了RNA可视化.
  • 将ExM与ISS,ISH和NGS相结合,可以提高RNA检测灵敏度和空间分辨率.
  • 空间转录组学提供了对基因表达,细胞类型和疾病机制的洞察.

结论:

  • 扩展显微镜显著提高了空间转录学性能.
  • 这些增强的技术提供了对细胞组织和疾病的更深入的理解.
  • 空间转录学具有巨大的潜力,可以为生物和医学研究取得突破.